| 玉米Mu转座子及其在反向遗传研究中的应用 |
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| 引用本文： | 崔庆新,李玉玲,杨美丽,冯兴跃.玉米Mu转座子及其在反向遗传研究中的应用[J].农业科技通讯,2010(1):35-38. |
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| 作者姓名： | 崔庆新 李玉玲 杨美丽 冯兴跃 |
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| 作者单位： | 1. 河南农业大学农学院,河南郑州,450002
2. 焦作市博爱县农业科学研究所,454450 |
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| 摘 要： | Mutator(Mu)是迄今为止发现活性最强的转座子。虽然对其作用机制尚不十分清楚,但已成功用于遗传研究,Mu转座子插入法也已成为克隆玉米基因的主要方法之一。目前B73玉米基因组测序已基本完成,并且在MaizeGDB数据库可以随时查询相关信息,利用反向遗传学方法研究基因的功能更为方便。本文简要介绍了Mu转座子的发现、类型、基本特征,重点介绍了Mu转座子在反向遗传研究中的应用。
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| 关 键 词： | 玉米 Mu转座子 反向遗传学 突变体 |
Mutator Transposon in Maize and Its Application in the Reverse genetics |
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| Authors: | CUI Qingxin LI Yuling Yang Meili FENG Xingyao |
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| Institution: | 1. College of Agronomy/a>;Henan Agricultural University/a>;Zhengzhou 450002/a>;China/a>;2. Boai Institute of Agricultural Science/a>;Boai 454450/a>;China |
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| Abstract: | Mutator (Mu) is the most active and mutagenic plant transposon discovered to date. Although the mechanism of Mu-element regulation was not thoroughly clear, it has been successfully used in the reverse genetics. Mu insertion has become one of the major ways in obtaining mutations and cloning genes in maize. Sequencing of the B73 maize genome is almost finished, and the sequencing data could be accessed in time by MaizeGDB. Using the method of reverse genetics to study gene function will be more convenient. ... |
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| Keywords: | maize Mutator transposon Reverse genetics mutant |
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