| 猪瘟细胞苗与犊牛血清中BVD/MD抗体的检测 |
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| 引用本文： | 程泽华.猪瘟细胞苗与犊牛血清中BVD/MD抗体的检测[J].山西农业大学学报(自然科学版),2003,23(2):143-144,154. |
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| 作者姓名： | 程泽华 |
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| 作者单位： | 山西省畜牧兽医学校,山西,太原,030024 |
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| 摘 要： | 猪瘟兔化牛睾丸细胞苗在生产中,常出现疫苗毒价不达标的问题。其主要的原因是在生产中使用了含BVD/MD抗体的犊牛血清。试验过程中,采用合格的疫苗与标准的BVD/MD抗血清中和,然后再加入标准BVD/MDV,接种BT细胞,结果出现CPE。证实了疫苗中的HCV与BVD/MD抗体发生了结合。故生产前对血清中BVD/MD抗体的检测是不可忽视的。
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| 关 键 词： | BVD/MD(牛病毒性腹泻/粘膜病) BVD/MDV(牛病毒性腹泻/粘膜病病毒) HCV(猪瘟病毒) CPE(细胞病变作用) BT细胞(牛鼻甲骨二倍体细胞) |
| 文章编号： | 1671-8151(2003)02-0143-02 |
Detection of the BVD/MD Antibody in Hog Cholera Cellular Vaccine and Calf Serum |
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| CHENG Ze-hua.Detection of the BVD/MD Antibody in Hog Cholera Cellular Vaccine and Calf Serum[J].Journal of Shanxi Agricultural University,2003,23(2):143-144,154. |
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| Authors: | CHENG Ze-hua |
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| Abstract: | During the production of hog cholera rabbit-adapeted bovine testis cellular vaccine, there occurred that the virulence of the vaccine has not attained its standard . The main reason is the use of calf serum with BVD/MD antibod-y. In the experiment , the eligible hog cholera rabbit-adapted bovine testis cellular vaccine neutralized the standard BVD/MD antiserum, then the standard BVD/MDV was added. The BT cells were inoculated in neutralized solution and resulted in CPE. This showed the combination of HCV in the hog cholera rabbit-adapted viral cellular vaccine with the BVD/MD antibody in the standard serum. It was necessary to detect BVD/MD antibody before producing the vaccine. |
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| Keywords: | BVD/MD BVD/MDV CPE HCV (hog cholera virus) BT cell |
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