山西省番茄叶霉病菌对多菌灵的抗性检测

从山西神池山区采集到 30个番茄叶霉病菌 Fulviafulva (Cooke)Ciferri]菌株 ,测得对多菌灵 (MBC)EC50的平均值为 0 0 978μg/mL ,最低抑制生长浓度 (MIC)值为 0 5 μg/mL。该区从未用过MBC ,以其EC50 的平均值定为敏感基线。从其它六个地区数十个县市采集分离得到 347个菌株 ,并测定了对MBC的敏感性。有 30 1个菌株属于抗性菌株 ,占总数的 86 7% ,并且 95 %以上的菌株EC50 >10 0 0 μg/mL。晋南和晋北地区的抗性频率最高 ,分别达到97 6 %和 10 0 % ;太原和吕梁地区相对低 ,但也分别达到 74 4 %和 79 6 %。抗性菌株经过多代无药培养后抗性程度没有明显变化。

Detection of Resistance to Carbendazim in Fulvia Fulva (Cooke) Ciferri From Shanxi Province

Sensitivity of 30 isolates of \%Fulvia fulva\% (Cooke)Ciferri,collected from a mountainous region of Shenchi in Shanxi Province,to carbendazim was tested.All minimum inhibitory concentration(MIC)of the chemical to the isolates was 0 5 μg/mL,and EC\-\{50\} value on average was 0 0978 μg/mL.These data could be taken as sensitive baseline because carbendazim has not been used ever before in that area.Sensitivity of 347 isolates collected from other 6 regions of Shanxi province to the chemical also was surveyed.301MBC-resistant isolates were found,and 95% of EC\-\{50\} values were higher than 1 000 μg/mL.It was impossible to measure MIC value.The resistance frequencies of north and south parts of Shanxi province were highest ,reaching to 97 6% and 100%;luliang and Taiyuan regions were relatively lower,being 74 7% and 79 6%.The resistance level of MBC-resistant isolates has not significantly declined after 20 generations growth on MBC-free PDA medium.