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PPV_(S-1)A株保存期研究及免疫原性测定
引用本文:张婉华,朱永军,张春玲,何锡忠,彭丽英.PPV_(S-1)A株保存期研究及免疫原性测定[J].山西农业大学学报(自然科学版),2014(3):213-215.
作者姓名:张婉华  朱永军  张春玲  何锡忠  彭丽英
作者单位:上海市农业科学院畜牧兽医研究所,上海佳牧生物制品有限公司,上海201106
摘    要:为了确保疫苗质量,保证生产用种毒的稳定性,将猪细小病毒PPVS-1A株的干毒和湿毒分别在-20℃和-70℃保存,每12个月取样一次,分别进行病毒含量测定、红细胞凝集价测定、乳鼠安全试验和豚鼠抗体检测。结果病毒含量均大于107.25;红细胞凝集价为1∶1024;乳鼠接种未见不良反应;豚鼠抗体检测HI均不低于1∶32。确定猪细小病毒PPVS-1A株湿毒在-20℃的保存期为24个月,在-70℃的保存期为48个月;猪细小病毒PPVS-1A株冻干毒在-20℃的保存期为36个月,在-70℃的保存期为60个月。猪细小病毒PPVS-1A株毒保存期长,具有良好的安全性和免疫效力。

关 键 词:猪细小病毒PPVs-1A株  -20℃保存  -70℃保存  保存期  免疫原性

Studies on Preservation Period and Immunogenicity of Porcine Parvovirus PPVs-1A Strain
Zhang Wanhua,Zhu Yongjun,Zhang Chunling,He Xizhong,Peng Liying.Studies on Preservation Period and Immunogenicity of Porcine Parvovirus PPVs-1A Strain[J].Journal of Shanxi Agricultural University,2014(3):213-215.
Authors:Zhang Wanhua  Zhu Yongjun  Zhang Chunling  He Xizhong  Peng Liying
Institution:1.Shanghai Jiamu Biologicals Limited Company ,Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106 ,China;)
Abstract:In order to guaranty the quality and toxic stability of virus vaccine,lyophilized virus and tamp-toxin of the porcine parvovirus PPVs-1A strain were store at-20℃ and-70℃,respectively.The virus content determination,red cell agglutination titer assay,safety test of suckling,and antibody determination of guinea pig were carried out every 12 months.The results showed the virus content was more than 10725,red cell agglutination titer was 1 ∶ 1024,no adverse reaction occurred after suckling inoculation,and Guinea pigs HI antibody was higher than 1 ∶ 32.therefore the storage period of tamp-toxin of the porcine parvovirus PPVs-1A strain was 24 months at-20℃ and 48 months at-70℃ ; the storage period of lyophilized virus of the porcine parvovirus PPVs-1 A strain was 36 months at-20℃ and 60 months at-70℃.
Keywords:The porcine parvovirus PPVs-1A strain  Preservation at-20 ℃  Preservation at-70℃  Preservation period  Immunogenicity
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