| 高灵敏度分光光度法测定乳酸氟哌酸的研究与应用 |
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| 引用本文： | 李红兵,李生泉,杨凤仙,黄晋果.高灵敏度分光光度法测定乳酸氟哌酸的研究与应用[J].山西农业大学学报(自然科学版),1996,16(2):194-196. |
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| 作者姓名： | 李红兵 李生泉 杨凤仙 黄晋果 |
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| 作者单位： | 山西省生物制品厂，山西农业大学中心实验室，山西农业大学校医院 |
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| 摘 要： | 本文较详细研究了乳酸氟哌酸—锆（Ⅳ）—四溴二氯萤光素三元络合物。在可见光谱区，创立了一种简便、快速、测定痕量乳酸氟哌酸的新方法。络合物的最大吸收峰在５５６ｎｍ处。表观摩尔吸光系数ε＝２．５８×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。乳酸氟哌酸含量在０～７０μｇ／１０ｍｌ范围内遵守比尔定律。方法用于兽药中乳酸氟哌酸的测定，结果令人满意。
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| 关 键 词： | 分光光度法，乳酸氟哌酸，四溴二氯萤光素 |
STUDY ON HIGHLY SENSITIVE SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACINI LACTAS AND ITS APPLICATION |
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| Li HongBing.STUDY ON HIGHLY SENSITIVE SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACINI LACTAS AND ITS APPLICATION[J].Journal of Shanxi Agricultural University,1996,16(2):194-196. |
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| Authors: | Li HongBing |
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| Abstract: | A new,simple and rapid spectrophotometric method for determination of trace Norfloxacini Lactas (NFL) with tetrabromodichloro-fluorescein(TBDCF) in the presebce of zirconium has been developed.The maxinum absorption of the complex of NFL-Zr (IV)-TBDCF is at 556 nm and its apparent molar absotptivity is 2.58×104L.mol-1.cm-1. Beer's law is obeyed br TBDCF in the range 0-70μg/10ml. Thg proposed method has been applied to determination of TBDCF in veterinary medicines with satisfactory results. |
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| Keywords: | Spectrophotometry Norfloxacini Lactas Tetrabromodichloro-fluorescein |
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