| 百脉根组织培养的研究 |
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| 引用本文： | 安骥飞,玉永雄.百脉根组织培养的研究[J].山西农业大学学报(自然科学版),2005,25(4):337-339. |
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| 作者姓名： | 安骥飞 玉永雄 |
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| 作者单位： | 西南农业大学,牧草与草食家畜重点实验室,重庆,北碚,400716 |
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| 基金项目： | 国家973项目（001CBl08905）,863项目（2001AA241164） |
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| 摘 要： | 以UM和MS为基本培养基,通过调整激素种类与浓度等培养条件,按正交实验设计的原则,摸索出建立百脉根组织培养体系的最佳条件:愈伤诱导最佳培养基为UM+2,4-D 2 mg.L-1+KT3 mg.L-1;最佳分化培养基为MS+NAA0.1 mg.L-1+6-BA 1 mg.L-1;最佳生根培养基为MS+NAA0.05 mg.L-1。
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| 关 键 词： | 百脉根(Lotuscorniculatus Linn) 组织培养 正交设计 |
| 文章编号： | 1671-8151（2005）04-0337-03 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-07-18 |
Studies on Tissue Culture of Lotus Corniculatus Linn |
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| AN Ji-fei.Studies on Tissue Culture of Lotus Corniculatus Linn[J].Journal of Shanxi Agricultural University,2005,25(4):337-339. |
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| Authors: | AN Ji-fei |
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| Abstract: | In this paper,MS and UM were selected as the base culture medium.According to the orthogonal design principle,the optimal culture conditions of Lotus corniculatus Linn were found through adjusting the varieties and concentration of hormones.The most appropriated culture medium in culture stages were UM+2,4-D 2 mg·L~(-1)+KT3 mg·L~(-1) for inducing callus,MS+NAA0.1 mg·L~(-1)+6-BA1 mg·L~(-1) for differentiation,and MS+NAA0.05 mg·L~(-1)for generating roots. |
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| Keywords: | Lotus corniculatus Linn Tissue culture Orthogonal design |
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