血清浓度对鸡胚盲肠上皮细胞原代培养的影响

为探讨细胞培养基中血清浓度对鸡盲肠上皮细胞体外培养的影响,分别用含0%、2.5%、5%和10%胎牛血清(FBS)的细胞培养液培养鸡胚盲肠上皮细胞,检测细胞活性;选择适宜的FBS浓度,并测定了该浓度条件下鸡盲肠上皮细胞的生长特性。结果表明:用含2.5%和5%FBS的培养基培养鸡胚盲肠上皮细胞,第4天细胞贴壁率达最高,分别为84%和80.33%,显著地高于无血清和10%FBS组(P<0.01/0.05),第7天仍均维持在76%以上,两者间除了第2天外,其余差异均不显著;用含2.5%FBS的细胞培养基进行鸡胚盲肠上皮细胞原代培养,第2~3天为适应阶段,第3~5天处于对数生长期,第5~9天细胞进入减速期,第10~11天再次进入对数生长期,第11天以后细胞进入衰退期,整个过程持续14 d左右。鸡胚盲肠上皮细胞培养的血清浓度以2.5%FBS为宜。

The Effects of Serum Concentration on Primary Culture of Cecum Epithelial Cells from Chicken Em-bryo

To investigate the effects of serum concentration on primary culture of cecum epithelial cells from chicken embryo,the cells were cultured in media including 0%,2.5%,5% and 10% FBS,respectively,then the cytoactivity was detected.The eligible concentration was selected and the growth characteristics were detected at that concentration.The results showed that when the cells were cultured in media including 2.5% and 5% FBS,the cell attachment rates were 84% and 80.33% respectively,and reached the peak on the 4th day,which were significantly higher than FBS-free and 10% FBS(P<0.01/0.05) and maintained above 76% on the 7th day.There was no singnificant difference except the 2nd day when cultured including 2.5% and 5% FBS.The adaptive phase was from the 2nd to the 3th day,the logarithmic growth phase was from the 3th to the 5th day,the deceleration phase was from the 5th to the 9th day,the second logarithmic growth phase was from the 10th to the 11th day,after the 11th day,the decline phase came,and the whole process lasted about 14 d.The eligible concentration of culture medium was containing 2.5% FBS.