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| 地肤子活性成分对山楂叶螨酯酶同工酶的影响 | |
| 引用本文： | 张慧荣,刘素琪,王海香,郝晓娟,莫海波,曹挥.地肤子活性成分对山楂叶螨酯酶同工酶的影响[J].山西农业大学学报(自然科学版),2011,31(4):321-325. |
| 作者姓名： | 张慧荣 刘素琪 王海香 郝晓娟 莫海波 曹挥 |
| 作者单位： | 1. 太原市园林局,山西太原,030001 2. 山西农业大学农学院,山西太谷,030801 3. 山西农业大学林学院,山西太谷,030801 |
| 基金项目： | 山西省青年基金，山西农业大学科技创新基金 |
| 摘 要： | 实验研究了地肤子活性成分KFCE-2处理后,山楂叶螨体内的酯酶同工酶的影响。从聚丙烯酰胺凝胶电泳结果来看,在处理组中,没有检测到新的其它酶带。并且其酯酶酶带E1、E2比对照组的颜色要浅,而且图谱面积小;而E3在处理的12h后完全消失。酯酶同工酶电泳图谱经UVP2凝胶成像系统扫描分析,从强度变化上可以看出,E2、E3带的活性从处理的4h(LANE-a')开始活性就在逐渐减弱,尤其是E3带的活性在处理的12h后(LANE-c')就完全被抑制。此外,在迁移率的变化上,处理组E1、E2带的迁移率有逐渐增大的趋势;E3带的迁移率在能观察到的两个时间内,有减小的趋势。这表明KFCE-2可能通过抑制山楂叶螨体内的酯酶同工酶的活性,并且通过特异性的结合,改变部分酯酶的活性结构,来降低其解毒代谢。这也表明了地肤子作为新型植物源农药具有良好的开发前景。 |
| 关 键 词： | 地肤 山楂叶螨 酯酶同工酶 |
Effects of the Chloroform Extracts of Kochia Scoparia (L.) Schrad on Several Enzyme Systems in Tetran ychus Viennensis |
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| ZHANG Hui-rong,LIU Su-qi,WANG Hai-xiang,HAO Xiao-juan,MO Hai-bo,CAO Hui.Effects of the Chloroform Extracts of Kochia Scoparia (L.) Schrad on Several Enzyme Systems in Tetran ychus Viennensis[J].Journal of Shanxi Agricultural University,2011,31(4):321-325. | |
| Authors: | ZHANG Hui-rong LIU Su-qi WANG Hai-xiang HAO Xiao-juan MO Hai-bo CAO Hui |
| Institution: | ZHANG Hui-rong1,LIU Su-qi2,WANG Hai-xiang3,HAO Xiao-juan2,MO Hai-bo2,CAO Hui2(1.Taiyuan Gardens Bureau,Taiyuan Shanxi 030001,China,2.College of Agriculture,Shanxi Agricultural University,Taigu Shanxi 030801,3.College of Foresty,China) |
| Abstract: | The changes of the activities of the esterase isoenzyme in Tetranychus Viennensis treated with chloroform extract of Kochia Scoparia(KFCE-2) were tested.The results of the polyacrylamide gel electrophoresis(PAGE) indicated that new band was not found in mites treated with KFCE-2,and the colors of the esterase bands E1 and E2 were lighter than that of the control group and the area of the maps were smaller.The band of E3 disappeared completely when treated after 12 h.When the electropherograms of PAGE were a... |
| Keywords: | Kochia Scoparia Tetranychus Viennensis Esterase isoenzyme |
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