| 碱性脂肪酶产生菌的发酵条件及酶学性质研究 |
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| 引用本文： | 张志东,茆军,唐琦勇,孙建,楚敏.碱性脂肪酶产生菌的发酵条件及酶学性质研究[J].新疆农业科学,2007,44(1):87-90. |
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| 作者姓名： | 张志东 茆军 唐琦勇 孙建 楚敏 |
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| 作者单位： | 新疆农科院微生物所,乌鲁木齐,830091 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 研究1株碱性脂肪酶产生菌L198发酵条件及酶学性质。结果表明,产酶发酵培养基为(%):豆饼粉1.0,玉米浆1.0,葡萄糖1.0,K2HPO40.5,NaNO30.5。500mL三角瓶装液50mL,30℃,180r/min摇床培养40h。酶学性质研究:最适反应温度为40℃,最适pH9.0;在pH8~10稳定。其热稳定性较好,在20~40℃放置6h后仍可以保持较好酶活力。金属离子Na+、K+对酶活有显著激活作用,而Cu2+对酶活有显著抑制作用,而K+、Mg2+、Fe+对酶活有抑制作用。
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| 关 键 词： | 碱性脂肪酶 产酶条件 酶学性质 |
| 文章编号： | 1001-4330（2007）01-0087-04 |
| 收稿时间： | 2006-06-27 |
| 修稿时间： | 2006年6月27日 |
Study on the Fermentation Condition and the Enzymatic Properties of an Alkaline Lipase-Producing Strain |
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| ZHANG Zhi-dong,MAO Jun,TANG Qi-yong,SUN Jian,Chu Ming.Study on the Fermentation Condition and the Enzymatic Properties of an Alkaline Lipase-Producing Strain[J].Xinjiang Agricultural Sciences,2007,44(1):87-90. |
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| Authors: | ZHANG Zhi-dong MAO Jun TANG Qi-yong SUN Jian Chu Ming |
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| Institution: | Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China |
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| Abstract: | An alkaline lipase-producing strain L198 was studied. The optimal medium composition and enzymatic properties were determined. The optimal medium composition for the lipase production is(%):soybean flour 1.0,corn milk 1.0,glucose 1.0, K2HPO4 0.5,NaNO3 0.5. Culture medium volumes for 50 mL flask were cultivated aerobically at 20℃ with an aeration rate of 180 r/min for 40 hours. The enzymatic properties were concluded. The optimum temperature and pH for it was 40℃ and 9.0.It was stable in the pH range from 8～10 and had good thermal stabilities-above 80% enzyme activity remained after incubated in the temperature range from 20～40℃ for 6 hours. The lipase activity was stimulated in the presence of metal ions such as Na+,K+,but was inhibited by Cu2+,K+,Mg2+,Fe+. |
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| Keywords: | alkaline lipase fermentation condition enzymatic property |
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