大白菜Ogura雄性不育系及保持系花药发育的细胞学观察

【目的】研究3个大白菜Ogura雄性不育系及保持系花药小孢子发育过程。【方法】对3对大白菜Ogura雄性不育系及其保持系花药发育进行细胞学观察。【结果】3个大白菜Ogura雄性不育系(OBY、OBK、OD5)的小孢子败育均发生在单核早期,在开花前完全败育。【结论】不育系绒毡层液泡化早于保持系,花粉母细胞减数分裂时不育系绒毡层细胞已开始退化,而保持系(BY、BK、D5)绒毡层自然解体,供给小孢子发育所需的营养物质和发育空间。

Morphological Studies on the Development of Anther of Three Ogura Cytoplasmic Male Sterile Lines and Their Maintainer Lines of Brassica rapa L.ssp.

【Objective】The development of anther microspore of three Ogura cytoplasmic male sterile(CMS) lines and their maintainer lines of Brassica rapa L.ssp.pekinensis were studied.【Method】The development of anther of three Ogura cytoplasmic male sterile(CMS) lines and their maintainer lines of Brassica rapa L.ssp.pekinensis were observed from the perspective of cytology.【Result】Microspore abortion of three Ogura CMS lines(OBY,OBK,OD5) have occurred in mononuclear microspore early,and anthers have aborted entirely before blossom.【Conclusion】Tapetum cell of three Ogura CMS lines formed vacuoles is earlier than their maintainer lines.Tapetum cell of three Ogura CMS lines has been degenerating at microsporocyte meiosis stage,and tapetum cell of their maintainer lines degenerated naturally,providing nutrients and space for microsporogenesis.