绵羊Myostatin前肽对成肌细胞的分化作用

【目的】 研究绵羊Myostatin前肽对C2C12细胞分化的影响。【方法】 构建并包装Myostatin前肽基因过表达慢病毒质粒,包装慢病毒,分别用LV-CMV-MSTN-flag-GFP和LV-CMV -GFP慢病毒感染C2C12细胞,检测重组慢病毒对C2C12细胞的感染能力。【结果】 LV-CMV -GFP感染组可见明显的长条状肌管,LV-CMV-MSTN-flag-GFP组只见少量肌管的形成,肌管融合率的测定。未处理组,对照组,试验组肌管融合率分别为5.53%、6.62%、9.38%,试验组即转染Myostatin前肽的肌管融合率显著高于对照组和未处理组(P＜0.05)。【结论】 Myostatin前肽基因的过表达抑制了Myostatin基因的表达,影响肌管的形成,抑制了分化的进程。

Effects of Sheep Myostatin Propeptide on Myoblast Differentiation

【Objective】 To study the effect of sheep myostatin propeptide on myoblast differentiation.【Method】 Myostatin cDNA Lentivirus vector plasmid was constructed and expressed in C2C12 cell and the ability of myostatin prepeptide to infect C2C12 cells was studied.【Result】 The LV-CMV -GFP infection group showed obvious long strips of myotubes, while LV-CMV-MSTN-flag-GFP group saw only a small amount of myotubes formed. To further determine the differentiation of myoblasts, myotube fusion rate was determined. The myotube fusion rates of were 5.53%, 6.62% and 9.38% respectively in the untreated group, the control group and the test group. The fusion rate of myotubes in the test group was significantly higher than that in the control group and the untreated group (P<0.05).【Conclusion】 The overexpression of Myostatin pro-peptide gene inhibited the expression of Myostatin gene, thus affecting the formation of myotubes and inhibiting the process of differentiation.