耐辐射丝状真菌F161的分离鉴定及其对锶的吸附特性

【目的】鉴定分离自新疆某辐射污染区的丝状真菌F161,研究其吸附锶特性。【方法】采用形态学观察、分子生物学鉴定及碳源利用实验鉴定菌种;采用压力试验和吸附试验研究特性,对锶的吸附测定采用ICP-MS,对放射性90锶的吸附的测定采用液闪检测仪。【结果】菌株F161鉴定为青霉属真菌;菌株F161能够耐受10 KGy剂量的钴源照射,对重金属离子Cr³⁺、Zn²⁺、Co²⁺、Pb²⁺、Hg²⁺均有较高耐受性。在pH 7.0,28℃下,伴随生长3~4 d,F161对Sr²⁺吸附效率最高能达到36.67%,对放射性90锶的吸附率为49.45%。【结论】F161是1株对放射性核素污染具有修复能力的土著丝状真菌,在有较高的重金属离子混合污染的放射性环境中具有一定的修复应用潜力。

Isolation and Identification of Filamentous Fungi F161 and Its Biosorption and Enrichment Characteristics of Strontiu

【Objective】 To identify the filamentous fungus F161 isolated from a radiation-contaminated area in Xinjiang and study its characteristics of strontium adsorption. 【Methods】 Morphological observation, molecular biology and carbon source utilization were used to identify F161 and the characteristics were studied by means of pressure test and adsorption experiment, and the adsorption of strontium was determined by ICP-MS, and the adsorption of radioactive strontium 90 was determined by liquid flash detector. 【Results】 The strain F161 was identified as Penicillium sp. by morphological, molecular biological identification and carbon source utilization experiments. The F161 strain could tolerate 10 KGy dose irradiation of radioactive cobalt, and also had high tolerance of heavy mental ions Cr³⁺, Zn²⁺, Co²⁺, Pb²⁺, Hg ²⁺. At pH 7.0 and 28℃ for 3-4 days with growth, the adsorption efficiency of Sr²⁺ by F161 was up to 36.67%, and the adsorption efficiency of radioactive strontium 90 was 49.45%. 【Conclusion】 F161 is an indigenous filamentous fungus with the ability to treat radionuclide contamination, which has the potential of remediation application in radioactive environment with high contamination of heavy metal ions.