| 适于核桃的RAPD-PCR反应体系的建立 |
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| 引用本文： | 王国安,张虎平,虎海防,牛建新,李西萍.适于核桃的RAPD-PCR反应体系的建立[J].新疆农业科学,2004,41(1):61-64. |
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| 作者姓名： | 王国安 张虎平 虎海防 牛建新 李西萍 |
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| 作者单位： | 1. 新疆林业科学院经济林所,新疆乌鲁木齐,830000
2. 石河子大学农学院园艺系,新疆石河子,832003 |
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| 基金项目： | 新疆自然科学基金(211003) |
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| 摘 要： | 采用CTAB法和改进的CTAB法,从叶片中提取核桃基因组DNA,比较其分离效果。结果表明,改进的CTAB法可有效去除细胞内多糖和多酚等杂质对模板DNA的污染。并以此为模板进行RAPD反应,对RAPD反应程序中的一些重要参数进行摸索和优化试验,建立适合于核桃的RAPD-PCR反应体系,以进行该树种的分子标记研究。
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| 关 键 词： | 核桃 DNA提取 RAPD |
| 文章编号： | 1001-4330(2004)01-0061-04 |
| 修稿时间： | 2003年11月10 |
Establishment of Optimal Reaction System of RAPD in Walnut |
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| WANG Guo-an,ZHANG Hu-ping,HU Hai-fang,NIU Jian-xin,LI Xi-ping.Establishment of Optimal Reaction System of RAPD in Walnut[J].Xinjiang Agricultural Sciences,2004,41(1):61-64. |
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| Authors: | WANG Guo-an ZHANG Hu-ping HU Hai-fang NIU Jian-xin LI Xi-ping |
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| Institution: | WANG Guo-an~1,ZHANG Hu-ping~2,HU Hai-fang~1,NIU Jian-xin~2,LI Xi-ping~2 |
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| Abstract: | Two methods(CTAB and reformed CTAB)were adopted to isolate genomic DNA of Walnut(Juglans rejia L.). The results show that the reformed CTAB is more effective to remove most of polysaccharides in the cytoplasmic and to inhibit the formation of oxidized polyphenolic compounds. Based on the genomic DNA extracted from Walnut, essential factors affecting the result of RAPD assay were tested and compared. An optimal reaction system suitable for the assay and usage of RAPD in Walnut was established. |
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| Keywords: | walnut DNA extract RAPD |
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