陆地棉光合和生理生化指标的耐盐性鉴定

目的 分析盐胁迫对陆地棉花铃期的光合和生理生化指标的影响,研究自然盐胁迫对陆地棉生理及产量的影响。建立综合得分(D值)公式,为棉花耐盐性的快速筛选和鉴定提供参考。方法 试验设计采用随机区组方式,2个处理分别为盐胁迫和对照,并重复两次进行,试验地为自然盐碱地,盐含量是根据各区组五点采样法取40 cm处的土样,测得处理1(盐胁迫)总含盐量为19 g/kg,处理2(对照)总含盐量为4 g/kg。结果 盐胁迫下光合指标明显下降,而不同的生理生化指标却有不同响应并且其相关性复杂;结合产量比较法得出盐胁迫下光合作用和POD活性越高,棉花品种越具有耐盐性,而MDA含量越低耐盐性越高。结论 利用隶属函数法和主成分分析法建立得到公式,D= 0.49F₁ + 0.16F₂ + 0.13F₃ + 0.12F₄ + 0.1F₅,可利用此公式筛选耐盐材料。

Identification of Salt Tolerance of Photosynthesis and Physiochemical Indexes of Gossypium hirsutum

Objective To explore the effects of salt stress on photosynthesis and physiochemical indexes of Gossypium hirsutum during the boll period, and to establish a comprehensive score (D value) formula, which might provide reference for rapid screening and identification of cotton salt tolerance.Method The whole experiment was designed in a randomized block mode. The two treatments were salt stress and control, and repeated twice. The other test sites were natural saline-alkali soils. The salt content was 40 cm according to the five-point sampling method of each block. At the soil sample, the total salt content of treatment 1 (salt stress) was 19 g/kg, and the total salt content of treatment 2 (control) was 4 g/kg. The effects of natural salt stress on the physiology and yield of gossypium hirsutum were studied.Results The results showed that the photosynthesis index decreased significantly under salt stress, but different physiological and biochemical indexes had different responses and their correlations were complex. The higher the photosynthesis and POD activity under salt stress were, the more salt tolerant cotton varieties were. The lower the MDA content, the higher the salt tolerance. The membership function method and principal component analysis method were used to establish the formula D=0.49F₁+0.16F₂+0.13F₃+0.12F₄+0.1F₅.Conclusion The comprehensive salt tolerance formula can be used to screen salt tolerant materials and used as a reference for rapid screening and identification of salt tolerance in cotton.