赤霉素对枣花繁殖生物学特性的影响

【目的】 研究赤霉素对枣花繁殖生物学特性的影响,为生产上赤霉素的科学应用提供理论依据。【方法】 以灰枣和骏枣的鲜花粉为材料,于盛花期喷施赤霉素(GA₃)处理,采用离体培养基,I-KI(碘-碘化钾)染色法,联苯胺-过氧化氢法测定,分析赤霉素对红枣花粉萌发和活力特性的影响。【结果】 低浓度(10~30 mg/L)赤霉素对灰枣和骏枣花粉萌发率、花粉管长度、花粉活力、花粉量和柱头可授性有较显著的促进作用。随着赤霉素浓度的升高,对花粉的萌发特性产生抑制作用;赤霉素处理后7 d的花粉萌发率和花粉活力跟对照没有显著差异。【结论】 枣花粉萌发、花粉管长度、花粉量和花粉活力特性,对外源赤霉素的响应表现为低浓度促进,高浓度抑制作用。

Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin

【Objective】 Spraying gibberellin in flowering stage is an important measure to promote fruit setting of jujube trees. To have a clearer understanding of its effect on the Biological characteristics of jujube flower reproductionwill provide a theoretical basis for the scientific application of gibberellin in production. 【Method】 The effects of gibberellin (GA₃) on the germination and vitality of jujube pollen were analyzed by using the in vitro culture medium, iodine potassium iodide staining and benzidine hydrogen peroxide method. 【Result】 By spraying different concentrations of gibberellin during the flowering stage of jujube, the pollen germination rate, pollen viability and pollen amount of Huizao and Junzao were significantly different from those of the control. Low concentration (10-30 mg/L) gibberellin significantly promoted pollen germination rate, pollen tube length, pollen viability, pollen quantity and stigma receptivity of the fruits. With the increase of gibberellin concentrations, the germination characteristics of pollen were inhibited; the pollen germination rate and pollen viability of 7 d after gibberellin treatment were not significantly different from those of the control. 【Conclusion】 The response of pollen germination, pollen tube length, pollen quantity and pollen activity to exogenous gibberellin was promoted by low concentration and inhibited by high concentration.