不同生长状况下新疆野苹果内源激素含量的变化

【目的】分析不同生长状况下新疆野苹果野果林在生长发育过程中内源激素的变化规律,为新疆野苹果保护研究提供科学依据。【方法】分别于不同发育周期、不同年限、不同生长状况的新疆野苹果叶片与果实鲜样,采用高效液相法测定IAA、GA₃、ABA、ZR、JA这5种激素的含量。【结果】从6~9月,正常的新疆野苹果叶片ZR、GA₃含量下降,JA、IAA、ABA含量上升;退化的新疆野苹果叶片ZR、JA、GA₃、IAA、ABA含量上升;退化和正常的果实ZR、JA、GA₃、IAA变化趋势相同,均呈下降的趋势。2018年相比于2017年,正常的新疆野苹果叶片ZR、JA、ABA含量上升,GA₃、IAA 含量下降;退化的新疆野苹果叶片ZR、ABA含量上升,GA₃、IAA 含量下降;正常和退化的果实内源激素含量变化趋势相同,均是JA 含量上升,ZR、GA₃、IAA、ABA 含量下降。在5种不同存活率的新疆野苹果中,得出100%存活率新疆野苹果林叶片的IAA、ZR、GA₃含量最高,20%存活率新疆野苹果林叶片的JA含量最高,10%存活率新疆野苹果林叶片的ABA含量最高,得出存活率越高,促进生长的激素含量也会越高。【结论】2种不同生长状况下,新疆野苹果5种内源激素含量在不同月份和不同年份表现出不同趋势。

Changes of Endogenous Hormones in Malus sieversii with Different Health Conditions

【Objective】 To analyze the changes of endogenous hormones during the growth and development of Xinjiang Malus sieversii forest under different health conditions, and to provide scientific basis for the research on the protection of Malus sieversii. 【Method】The contents of IAA, GA₃, ABA, ZR, JA in leaves and fruits of Xinjiang Malus sieversii with different development cycles, different years and different health conditions were determined by high performance liquid chromatography (HPLC). 【Result】From June to September, the contents of ZR and GA₃ in the leaves of normal Malus sieversii decreased, and the contents of JA, IAA and ABA increased. The contents of ZR, JA, GA₃, IAA and ABA in the degraded Malus sieversii increased. The normal fruits ZR, JA, GA₃, and IAA showed the same trend, and all showed a downward trend. Compared with those in 2017, the contents of ZR, JA and ABA in the leaves of normal Xinjiang Malus sieversii increased, and the contents of GA₃ and IAA decreased. The content of ZR and ABA in the leaves of degraded Malus sieversii increased, and the contents of GA₃ and IAA decreased. Normal and degraded endogenous hormone contents of fruits showed the same trend, and the content of JA increased, and the contents of ZR, GA₃, IAA and ABA decreased. Among the five Malus sieversii with different survival rates, the 100% survival rate of the leaves of Malus sieversii forest was the highest in IAA, ZR and GA₃, and the 20% survival rate of Malus sieversii forest leaves was the highest, 10% survival rate of Malus sieversii forest leaves was the highest. It can be seen that the higher the survival rate, the higher the hormone content that promotes growth. 【Conclusion】The contents of five endogenous hormones in Malus sieversii under two different growth conditions showed different trends in different months and years.