蛙皮素样肽家族及其受体氨基酸序列信息学比较分析

【目的】研究反刍动物蛙皮素样肽家族多肽及其受体的保守性。为不同动物,特别是反刍动物这2种蛙皮素样多肽及其受体蛋白抗原设计和活性多肽筛选等研究提供参考。【方法】采用生物信息分析学的方法,通过UniProt数据库比较牛的蛙皮素样肽家族胃泌素释放肽(Gastrin-releasing peptide, GRP)和神经介素B(Neuromedin B, NMB)2种多肽及其特异性受体GRP-R和NMB-R与其他动物氨基酸序列的差异性。【结果】13种动物成熟GRP多肽C-端的8个氨基酸序列完全相同,牛GRP氨基酸序列与绵羊、猪和豚鼠最高,分别为88.7%、77.8%和77.8%,与其他动物相似性低于74.1%。10种动物成熟NMB多肽C-端10个氨基酸序列完全相同。牛GRP-R与狗、马和猪等相似性最高,分别为96.1%、94.3%和94.0%,牛NMB-R与猪、人和马相似性最高,分别为92.3%、91.5%和91.0%;牛GRP-R与NMB-R相似性仅为62.2%。【结论】GRP和NMB的C-端氨基酸序列在不同动物之间均具有高度的保守性,而N-端为变化区域,且GRP-R和NMB-R氨基酸序列在动物之间保守性较高。

Informatics Analysis of Amino Acid Sequences of Bombesin-like Peptides and Their Receptors

【Objective】 To understand the bombesin-like peptide (BLP) family and the conservation of their receptors in ruminants. 【Method】In this study, biological information analysis was used to compare the amino acid sequences of bovine bombesin-like peptide family gastrin releasing peptides (Gastrin-releasing peptide, GRP) and Neuromedin B (NMB)) and their specific receptors GRP-R and NMB-R with other animal amino acid sequences by UniProt database. 【Result】The results of bioinformatics analysis showed that 8 amino acid sequences of C-terminal of mature GRP in 13 animals were identical, and the amino acid sequences of bovine GRP had high similarity 88.7%, 77.8% and 77.8% with sheep, pigs and guinea pigs, respectively, moreover, the similarity with other animals was less than 74.1%. The 10 amino acids at the C-terminal of mature NMB in 10 animals were identical. Bovine GRP-R had the highest similarity with dogs, horses and pigs, 96.1%, 94.3% and 94.0%, respectively. Bovine NMB-R had the highest similarity with pigs, humans and horses, 92.3%, 91.5% and 91.0%, respectively. The similarity between GRP-R and NMB-R was only 62.2% in cattle. 【Conclusion】 The above analysis results indicated that the C-terminal amino acid sequences of both GRP and NMB were highly conserved among animals, while the N-terminal was the variable region, and the amino acid sequences of GRP-R and NMB-R were highly conserved among animals. The results of this bioinformatics analysis can be used as a reference for the study on the antigen design and selection of active polypeptides of these two kinds of BLP in different animals, especially in ruminants.