| 新疆野生冰草抗旱功能基因BADH的克隆及表达载体的构建 |
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| 引用本文： | 贾纳提,李斯深,朱昊,齐照良,刘梦,别尔肯·伊斯马伊诺夫,马海燕.新疆野生冰草抗旱功能基因BADH的克隆及表达载体的构建[J].新疆农业科学,2012(12):2269-2275. |
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| 作者姓名： | 贾纳提 李斯深 朱昊 齐照良 刘梦 别尔肯·伊斯马伊诺夫 马海燕 |
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| 作者单位： | 新疆畜牧科学院草业研究所;山东农业大学;哈萨克斯坦农业部畜牧与饲草饲料研究所 |
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| 基金项目： | 科技支疆项目“新疆荒漠逆境植物抗旱功能基因的筛选与应用”(20084J0102-05);国际合作项目“新疆天然草地植被恢复与重建技术平台合作研究”(2008DFA30840) |
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| 摘 要： | 【目的】克隆新疆野生冰草抗旱相关基因甜菜碱醛脱氢酶(betaine aldehyde dehydrogenase,BADH)基因,为进一步研究其抗旱功能奠定基础。【方法】利用同源克隆法和RACE方法扩增获得新疆草原区强抗旱植物沙生冰草抗旱相关基因BADH,并将其构建到植物表达载体pBI121上。【结果】克隆获得的新疆野生沙生冰草抗旱相关基因BADH全长1422 bp,编码区序列全长为1182 bp,GenBank注册号为GU181396.1,并成功构建了植物过表达载体。
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| 关 键 词： | 冰草 甜菜碱醛脱氢酶 基因 克隆 |
Cloning and Plant Expression Vector Construction of BADH from Wheatgrass in Xinjiang |
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| Institution: | Jianat1,LI Si - shen2,ZHU Hao1,JI Zhao - liang2,LIU Meng1,Belke Ismayilov3,MA Hai - yan1 (1.Pratacultural Research Institute,Xinjiang Academy of Animal Sciences,Urumqi 830000,China;2. Shandong Agricultural University,Taian SHANG dong 271018,China;3.Animal Husbandry and Forage Feed Research Institute,Agricultural Ministry of Kazakhstan,Alma - Ata 050000,Kazakhstan) |
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| Abstract: | Objective]For further study of the drought resistance ability of BADH,we cloned the drought resistance related gene BADH from wheatgrass in Xinjiang.Method]Homologous cloning method and RACE amplification method were applied to clone the drought resistance related gene BADH from wheatgrass in Xinjiang.The plant expression vector of BADH was constructed and named pBI121 - BADH.Result] The sequence of BADH obtained from wheatgrass in Xinjiang clone was 1 422 bp,the ORF was 1 182 bp,GenBank registration number is GU181396.1,and the plant expression vector of BADH was constructed successfully. |
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| Keywords: | wheatgrass bataine aldehyde dehydrogenase(BADH) gene clone |
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