刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析

【目的】 研究刺山柑乙醇提取物正丁醇萃取部位的抗炎镇痛活性。【方法】 以总黄酮提取量为指标采用超声波辅助提取法并结合响应面优化乙醇提取物最佳工艺,将乙醇提取物浸膏通过萃取得到正丁醇部位。采用二甲苯致小鼠耳廓肿胀、小鼠腹腔毛细血管通透性分析正丁醇萃取部位抗炎效果;以热板法和醋酸扭体考察正丁醇萃取部位镇痛效果。【结果】 正丁醇部位高剂量组、中剂量组对二甲苯致小鼠耳廓肿胀有明显抑制作用(P＜0.01);正丁醇部位高剂量组、中剂量组对小鼠毛细血管通透性有明显抑制作用(P＜0.01);正丁醇部位高剂量组、中剂量组可显著提高小鼠热板痛阈值,正丁醇部位高剂量组、中剂量组能明显减少醋酸所致小鼠扭体次数(P＜0.01)。【结论】 刺山柑果实醇提物正丁醇部位提取物具有潜在的抗炎镇痛作用。

Anti-inflammatory and Analgesic Effects of Alcohol Extracts from Capers Fruits and N-butanol Extracts

【Objective】 To investigate the anti-inflammatory and analgesic activity of ethanol extracts of Capparis spinosa and n-butanol. 【Method】 Using ultrasonic-assisted extraction method combined with response surface to optimize the optimal ethanol extraction process with the total flavonoids extraction as the index, extract the ethanol extract to obtain the n-butanol fraction. The anti-inflammatory effect of the n-butanol extraction site was investigated using xylene-induced swelling of the auricle of mice and the capillary permeability of the abdominal cavity of the mice; the analgesic effect of the n-butanol extraction site was examined by hot plate method and twisted acetate. 【Results】 The high-dose and medium-dose groups of n-butanol significantly inhibited the auricle swelling induced by xylene in mice (P<0.01); Permeability has a significant inhibitory effect (P<0.01); high-dose and mid-dose groups in the n-butanol area could significantly increase the hot plate pain threshold in mice, and high-dose and middle-dose groups in the n-butanol area could significantly reduce the number of writhing in mice induced by acetic acid (P<0.01). 【Conclusion】 The n-butanol extract of Capparis spinosa fruit has potential anti-inflammatory and analgesic effects.