猪源大肠杆菌耐药性及相关耐药基因检测

【目的】 研究新疆阿克苏地区某规模化猪场猪源大肠杆菌对临床常用抗菌药物的耐药情况及相关耐药基因携带情况,分析耐药表型与耐药基因之间的关系。【方法】 采用琼脂稀释法,对分离鉴定的443株猪源大肠杆菌进行猪场常用6大类13种抗菌药物最小抑菌浓度的测定。采用PCR方法,检测13种抗菌药物相应的4大类10种耐药基因。【结果】 该猪场猪源大肠杆菌对被检的8种抗菌药物耐药率超过70.0%,其中对恩诺沙星、链霉素、大观霉素、金霉素、土霉素的耐药率高达90.0%以上,对阿米卡星、安普霉素、头孢噻呋、多黏菌素E具有较好的敏感性,耐药率未超过25.0%;多药耐药结果以7~9耐为主,占63.6%。除tetK外,对被检的其他9种耐药基因均有检出,以携带floR(66.4%)和ant(3″)-Ia(66.4%)耐药基因为主。【结论】 新疆阿克苏地区某规模化猪场猪源大肠杆菌至少对被检的1种抗菌药物表现为耐药,且多药耐药情况严重,耐药谱广,耐药基因携带率高,应从基因水平对耐药大肠杆菌进行传播机制的监测。

Investigation on Drug Resistance and Detection of Related Resistance Genes of Escherichia coli from Pigs

【Objective】 To understand the resistance to common antimicrobial agents and the carrying of related drug resistance genes of E.coli derived from swine in a large scale pig farm in Aksu region of Xinjiang and to explore the relationship between drug-resistant phenotypes and drug-resistant genes.【Method】 In this study, the agar dilution method was used to determine the minimum inhibitory concentration of 13 kinds of antimicrobial agents commonly used in pig farms for 443 strains of Escherichia coli isolated and identified from pigs. And the PCR method was used to detect 10 resistance genes in 4 classes of 13 antimicrobial agents.【Result】 The resistance results showed that the resistance rate of E.coli from swine to 8 kinds of antibiotics was over 70.0%, and the resistance rate to enrofloxacin, streptomycin, spectinomycin, aureomycin and oxytetracycline was over 90.0%, and it had good sensitivity to amikacin, ampomycin, cefotaxime, polymyxin E, the drug resistance rate was not more than 25.0%. The multidrug resistance results was concentrated in 7-9, accounting for 63.6%. The results of drug resistance gene detection showed that: with the exception of tetK gene, all the other 9 drug resistance genes were detected, mainly carrying floR (66.4%) and ant (3 ")-Ia (66.4%)resistance genes. 【Conclusion】 E.coli derived from swine in a large-scale pig farm in Aksu region, Xinjiang, showed resistance to at least one antibacterial drug tested, the multi-drug resistance is serious, the drug resistance spectrum is wide, and the drug resistance gene carrying rate is high. It is suggested that the transmission mechanism of drug-resistant E. coli should be monitored at the gene level, and in addition, the relevant departments should strengthen the standardized use of antibacterial drugs.