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牛病毒性腹泻病毒结构蛋白基因的克隆及其B细胞抗原表位的预测
引用本文:何延华,黄新,薄新文,钟发刚,韩猛立,陈南颖,王新华.牛病毒性腹泻病毒结构蛋白基因的克隆及其B细胞抗原表位的预测[J].新疆农业科学,2012,49(2):310-317.
作者姓名:何延华  黄新  薄新文  钟发刚  韩猛立  陈南颖  王新华
作者单位:新疆农垦科学院/新疆兵团绵羊繁育生物技术重点实验室,新疆石河子,832000
基金项目:新疆兵团青年创新资金项目(2010JC22);国家自然科学基金项目(30960286,30960281);新疆生产建设兵团博士资金课题(2007JC15)
摘    要:【目的】以免疫信息学和反向疫苗学为理论根据,应用生物信息学相关软件和方法,对BVDV结构蛋白(Structural Protein,SP)的二级结构的不同方面及其B细胞表位进行了预测,综合评价了BVDV结构蛋白的抗原特异性细胞表位,并计算出一些潜在的抗原位点。【方法】利用RT-PCR扩增法获得牛病毒性腹泻病毒结构蛋白序列,通过克隆、测序分析结构蛋白基因的基因组序列和蛋白序列,采用DNAStar Protean软件对结构蛋白的二级结构、可塑性、亲水性、表面可及性及抗原指数等参数进行综合分析,并预测其B细胞的抗原表位分布。【结果】P14、gp48和gp53蛋白含有的细胞优势抗原表位较多,有的甚至有可能成为优势抗原表位或对优势抗原表位有协同作用。【结论】与已鉴定的B细胞抗原表位相比,试验所用方法预测的结果有较高的准确度,是一种较为先进的表位研究方法。

关 键 词:牛病毒性腹泻病毒  结构蛋白  B细胞表位  二级结构  反向疫苗学

Cloning of BVDV Structural Protein Gene and Predictions of B Lymphocyte Epitopes of Structural Protein
HE Yan-hua , HUANG Xin , BO Xin-wen , ZHONG Fang-gang , HAN Meng-li , CHEN Nan-ying , WANG Xin-hua.Cloning of BVDV Structural Protein Gene and Predictions of B Lymphocyte Epitopes of Structural Protein[J].Xinjiang Agricultural Sciences,2012,49(2):310-317.
Authors:HE Yan-hua  HUANG Xin  BO Xin-wen  ZHONG Fang-gang  HAN Meng-li  CHEN Nan-ying  WANG Xin-hua
Institution:(Xinjiang Academy of Agricultural and Reclamation Sciences/Key Laboratory of Sheep Breeding Biology Technology of Xinjiang Production and Construction Corps,Shihezi Xinjiang 832000,China)
Abstract:【Objective】On the basis of immunological bioinformatics and reverse vaccinology,the secondary structure of the BVDV structural protein(SP) and its B lymphocyte epitopes with specificity of antigen were predicted and evaluated comprehensively,and some potential antigenic determinants were worked out from the SP gene sequence of the strain with related software and methods of bioinformatics.【Method】The structural protein(SP) gene of the bovine viral diarrhea virus(BVDV) was amplified by RT-PCR and then sequenced.The secondary structure,flexibility,hydrophilicity,accessibility and antigenicity of pl were analyzed by using DNA Star Protean software,and the B cell epitopes were predicted.【Result】The results showed that there were several domains in P14,gp48 and gp53 which belonged to predominant B cell epitopes and a few of B cell epitopes were in gp25.【Conclusion】 Compared with the epitopes that have been published,the results predicted by this method had high reliability and this method was a more advanced epitope research approach.
Keywords:Bovine Viral Diarrhea Virus(BVDV)  structural protein  B cell epitope  secondary structure  reverse vaccinology
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