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鸡腿菇单孢分离和单孢杂交的研究
引用本文:张树强,卫彩红,胡建伟.鸡腿菇单孢分离和单孢杂交的研究[J].新疆农业科学,2012,49(1):190-194.
作者姓名:张树强  卫彩红  胡建伟
作者单位:塔里木大学植物科学学院,新疆阿拉尔,843300;塔里木大学生命科学学院,新疆阿拉尔,843300;塔里木大学食用菌研究所,新疆阿拉尔,843300
基金项目:新疆生产建设兵团塔里木盆地生物资源保护利用重点试验室开放课题(BR0802);新疆生产建设兵团工业科技攻关项目(2009GG38)
摘    要:目的]杂交育种被应用在各个物种之间.选育高品质,高产量的菌种.方法]单孢分离技术是杂交育种行之有效的育种手段之一,近年来国内已开展这方面的研究.试验利用简单的仪器,设计一种适合鸡腿菇孢子收集和单孢分离的方法.结果]鉴定挑选出35个单核菌株进行配对杂交,经染色镜检、拮抗试验、瓶栽试验,获得48个杂交新菌株.结论]应用单孢分离技术对鸡腿菇进行杂交行之有效.

关 键 词:鸡腿菇  菌丝长势  菌丝长速  杂交
收稿时间:2012-01-25

Studies on Single-Spore Isolation and Hybridize of Coprinus comatus
ZHANG Shu-qiang , WEI Cai-hong , HU Jian-wei.Studies on Single-Spore Isolation and Hybridize of Coprinus comatus[J].Xinjiang Agricultural Sciences,2012,49(1):190-194.
Authors:ZHANG Shu-qiang  WEI Cai-hong  HU Jian-wei
Institution:1.College of Plant Science,Tarim University,Alar Xinjiang 843300,China;2.College of Life Science,Tarim University,Alar Xinjiang 843300,China;3.Edible Fungi Research Institute of Tarim University,Alar Xinjiang 843300,China)
Abstract:【Objective】The purpose of this project is to breed a high quality and yield fungoid.Hybridization has been taken to all of the species.【Method】Single-Spore Isolation is one of the best way for hybridization,and many researchers have made studies in this field.With the aid of the simplest apparatus,a practical and simplest method of single-spore isolation and hybridization of Coprinus comatus was designed.【Result】After appraisal,35 Single-Spore mycelia were chosen for hybridization.After examination under coloration microscope,test of antagonistic lines,outgrowth generation in bottle,at last 48 new strains of filial generation were got.【Conclusion】Using single-spore isolation to hybridize Coprinus comatus is effective.
Keywords:Coprinus comatus  growth potential  growth rate  hybridize
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