引起新疆向日葵列当茎基腐病的镰刀菌分离与鉴定

【目的】向日葵列当在新疆严重发生,从田间自然死亡的列当植株上分离致病菌并进行鉴定,为新疆列当生物防治提供菌种来源。【方法】采用组织分离法分离采集的样品,采用形态学及分子生物学方法对采集的镰刀菌进行鉴定。【结果】从587个生病的向日葵列当样品上共计分离获得377个分离物,其中镰刀菌234个,占总分离物的62.07%;丝核菌114个,占30.24%;腐霉菌6个,占1.59%;其它23个,占6.10%。在镰刀菌中鉴定出了3个种,分别为尖孢镰孢Fusarium oxysporum、茄病镰孢Fusarium solani和Fusarium cerealis。【结论】镰刀菌是引起新疆向日葵列当的主要致病菌,为向日葵列当生防菌的进一步筛选提供了菌株来源。

Isolation and Identification of Pathogen of Orobanche cumana Stem-rot Disease in Xinjiang

【Objective】Orobanche cumana occurs seriously in Xinjiang.Pathogens were isolated from the disease Orobanche cumana plant and were identified,which can be used as source strains for biological control of Orobanche cumana.【Method】Tissue isolation was used to isolate diseased plant sample,morphology and molecular biology were used to identify Fusarium.【Result】A total of 587 samples of diseased Orobanche plant were collected and 377 isolates were obtained.According to morphological features of culture,234 of these,62.07% of the total isolates,belong to Fusarium;114 of these,30.24% of the total isolates,belong to Rhizotonia;6 of these,1.59% of the total isolates,belong to Pythium and 23 of these,6.46% of the total isolates,belong to others.Three Fusarium species identified were Fusarium oxysporum,F.solani and F.cerealis.【Conclusion】The main pathogens of Orobanche cumana were Fusarium in Xinjiang,which was a new strain source provided for biological control of Orobanche cumana.