| 甜菜离子注入诱变早熟突变体的RAPD分子标记筛选 |
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| 引用本文： | 曲延英,王燕飞,高文伟,沙红,张立明,张亚静,朱晓平,李道远.甜菜离子注入诱变早熟突变体的RAPD分子标记筛选[J].新疆农业科学,2005,42(4):287-289. |
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| 作者姓名： | 曲延英 王燕飞 高文伟 沙红 张立明 张亚静 朱晓平 李道远 |
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| 作者单位： | 1. 新疆农业大学农学院,新疆乌鲁木齐 830052
2. 新疆农科院经作所,新疆乌鲁木齐 830000 |
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| 基金项目： | 自治区高技术发展计划项目“甜菜主要农艺性状及离子注入诱变性状的分子标记助育种”（200415114） |
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| 摘 要： | 以离子注入诱变技术获得的甜菜早熟突变体W11和未做诱变处理的7208为对照材料,提取DNA并进行RAPD分析,选取800条RAPD随机引物对W11和7208进行扩增,结果表明,W11和7208之间在DNA水平上存在差异。从800条随机引物中筛选到45条在W11和7208中存在差异,存在差异的引物占所用引物数的5.6%,表明W11和7208种的相似性很高。该研究利用W11和7208做亲本构建分子标记作图群体,进而定位早熟性状基因或QTL奠定了基础。
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| 关 键 词： | 甜菜 离子注入 早熟突变体 分子标记 |
| 文章编号： | 1001-4330（2005）04-0287-03 |
| 收稿时间： | 2005-05-20 |
| 修稿时间： | 2005年5月20日 |
Sifting molecule mark of RAPD by sugar beet ion impregnating into mutagenic early-maturing mulant |
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| QU Yan-ying,WANG Yan-fei,GAO Wen-wei,Sha Hong,ZHANG Li-ming,ZHANG Ya-jing,ZHU Xia-ping,LI Dao-yuan.Sifting molecule mark of RAPD by sugar beet ion impregnating into mutagenic early-maturing mulant[J].Xinjiang Agricultural Sciences,2005,42(4):287-289. |
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| Authors: | QU Yan-ying WANG Yan-fei GAO Wen-wei Sha Hong ZHANG Li-ming ZHANG Ya-jing ZHU Xia-ping LI Dao-yuan |
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| Abstract: | In our experiment,DNA was extracted and RAPD was analyzed by using sugar beet early - maturing mutant W11 obtained by impregnating ion with mutagenic technology and 7208 which has not been mutagenized as the control material.The W11 and 7208 increased by randomly selecting 800 primers of RAPD.The result indicated that there was difference on DNA level between W11 and 7208.There was difference in W11 and 7208 of 45 primers which had randomly beeb sifted from 800 ones.The number of the primers with difference was up to 5.6% of the total. It indicated that W11 was very much similar to 7208. The study was conducted to establish the parent structure molecule mark map colong and also establish the early - maturing properties gene or QTL foundation. |
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| Keywords: | sugar beet ion impregnate early - maturing mutant molecule mark |
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