富氢水对干旱胁迫下大麦种子萌发的影响

目的]研究富氢水对干旱胁迫下大麦种子萌发的影响,分析富氢水在干旱应答中的作用.方法]以大麦品种新啤6号为试材,采用不同浓度(0、25％、50％、75％、100％)富氢水浸种处理,测定半致死聚乙二醇-6000浓度下大麦种子发芽特性、渗透调节物质含量、抗氧化酶活性及丙二醛含量变化.结果]25％及50％富氢水浸种能明显...

Effects of Hydrogen-Rich Water on Barley Seed Germination under Drought Stress

【Objective】 To study the effects of hydrogen-rich water (HRW) on barley seeds germination under drought stress and clarify the role of HRW in drought response. 【Method】 The changes of germination characteristics, osmotic regulation substance content, antioxidant enzyme activity and malondialdehyde content of barley seeds (Xinpi 6) under the concentration of semi-lethal polyethylene glycol-6000 were measured by soaking seeds with different concentrations (0,25%, 50%,75%,100%).【Result】 25% and 50% HRW soaking seeds could significantly improve the germination quality of barley seeds under drought stress. Under the above concentration of HRW treatment, the germination potential, germination rate and germination index increased significantly (P<0.05); the contents of soluble protein, soluble sugar and free proline increased to different degrees; the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) also increased to different levels and the expression of malondialdehyde (MDA) decreased significantly (P<0.05). 【Conclusion】 The appropriate concentration of HRW can enhance the tolerance of barley seeds to drought stress by enhancing the ability of osmotic regulation and antioxidation.