| 离体培养下氮素浓度对小麦籽粒HMW-GS表达动态和含量的影响 |
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| 引用本文： | 周洪华,董心久,魏凌基,茹思博,朱继正.离体培养下氮素浓度对小麦籽粒HMW-GS表达动态和含量的影响[J].新疆农业科学,2007,44(2):187-193. |
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| 作者姓名： | 周洪华 董心久 魏凌基 茹思博 朱继正 |
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| 作者单位： | 1. 石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子,832003;中国科学院研究生院,北京,100039
2. 石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子,832003 |
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| 摘 要： | 利用小麦穗离体培养技术和SDS-PAGE技术,研究了不同氮素浓度处理下小麦籽粒高分子量谷蛋白亚基(HMW-GS)的表达和积累规律。研究显示:氮素浓度不影响HMW-GS组成和表达顺序,各处理下HMW-GS的表达顺序均依次为亚基8、亚基7、亚基2和亚基12。但各亚基的表达时间与含量明显受氮素浓度影响:低氮浓度处理与高氮浓度处理相比,亚基8、亚基7、亚基2和亚基12的表达时间分别从花后121、5和18 d提前到花后6和9 d;HMW-GS的含量均与氮素浓度呈正相关,特别是亚基2不仅与氮素浓度呈极显著正相关,且影响亚基12对氮素的响应。
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| 关 键 词： | 离体培养 小麦 氮素浓度 HMW-GS 表达动态 积累规律 |
| 文章编号： | 1001-4330（2007）02-0187-07 |
| 收稿时间： | 2006-09-25 |
| 修稿时间： | 2006年9月25日 |
Effect of Different Nitrogen Concentration on Expression Trend and Content of HMW-GS in Wheat Kernel |
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| ZHOU Hong-hua,DONG Xin-jiu,WEI Li-ji,Ru Sibo,ZHU Ji-zheng.Effect of Different Nitrogen Concentration on Expression Trend and Content of HMW-GS in Wheat Kernel[J].Xinjiang Agricultural Sciences,2007,44(2):187-193. |
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| Authors: | ZHOU Hong-hua DONG Xin-jiu WEI Li-ji Ru Sibo ZHU Ji-zheng |
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| Institution: | 1. Key Laboratory of Oasis Eco - agriculture of Xinjiang Production and Construction Corps, College of Agronomy, Shihhotze University , Xinjiang 832003, China ;2. Gruduate School, Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | Development and accumulation law of HMW-GS was studied in different nitrogen content by SDS-PAGE technology in wheat in vitro.The results showed that nitrogen concentration didn't affect the composition and the expression order of HMW-GS.The expression order of HMW-GS was subunit 8,subunit 7,subunit 2 and subunit 12 in turn in different nitrogen concentration,but the expression time and content of each subunit were affected by nitrogen concentration obviously.The expression time of subunit 8,subunit 7,subunit 2 and subunit 12 was advanced ahead of time after flower 6 d and 9 d from the time after flower 12 d,15 d,18 d individually in higher nitrogen content.A significant positive correlation was found between the content of HMW-GS and the different nitrogen concentration,especially the subunit 2.The subunit 2 was not only extremely remarkable positive correlation with the nitrogen concentration,but also affect the nitrogen response of the subunit 12. |
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| Keywords: | HMW-GS |
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