| 扩展莫尼茨绦虫膜联蛋白B3基因组织表达差异分析 |
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| 引用本文： | 王正荣,赵文娟,王新华,薄新文.扩展莫尼茨绦虫膜联蛋白B3基因组织表达差异分析[J].新疆农业科学,2011(10):1864-1869. |
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| 作者姓名： | 王正荣 赵文娟 王新华 薄新文 |
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| 作者单位： | 新疆农垦科学院新疆绵羊重点繁育实验室; |
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| 基金项目： | 国家科技部“973”前期研究专项(2006CB708512); 新疆生产建设兵团杰出青年创新资金专项(2011CD005); 兰州兽医研究所家畜疫病病原生物学国家重点实验室2011年度开放基金课题(SKLVEB2011KFKT008) |
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| 摘 要： | 【目的】探索膜联蛋白B3(Annexins B3)基因在扩展莫尼茨绦虫生长发育中的作用。【方法】研究以beta-Tubulin基因作为内参基因,采用SYBR Green real-time RT-PCR方法检测该基因在扩展莫尼茨绦虫四个不同发育阶段即头节、幼节、成节、孕节中的表达差异。【结果】标准曲线分析显示,Annexins B3和beta-Tubulin基因的Ct值与阳性质粒的浓度均呈良好的线性关系,相关系数均大于0.991,溶解曲线分析表明,产物为特异的单峰,具有较高的特异性。【结论】膜联蛋白B3基因在虫体个发育阶段中的表达丰度存在差异,从高到低依次为孕节、头节、成节、幼节。
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| 关 键 词： | Annexins B3 扩展莫尼茨绦虫 RT-PcR 差异表达 |
Analysis of the Differential Expression of the PICKI Gene in Moniezia expansa |
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| WANG Zheng-rang,ZHAO Wen-juan,WANG Xin-hua,BO Xin-wen.Analysis of the Differential Expression of the PICKI Gene in Moniezia expansa[J].Xinjiang Agricultural Sciences,2011(10):1864-1869. |
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| Authors: | WANG Zheng-rang ZHAO Wen-juan WANG Xin-hua BO Xin-wen |
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| Institution: | WANG Zheng-rang,ZHAO Wen-juan,WANG Xin-hua,BO Xin-wen (Xinjiang Academy of Agricultural and Reclamation Sciences,Key Laboratory of Sheep Breeding Biology Technology of Xinjiang Production and Construction Corps,Shihezi Xinjiang 832000,China) |
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| Abstract: | 【Objective】The program aimed to explore the Annexins B3 gene in the development of Moniezia expansa.【Method】This study developed a SYBR green real-time fluorescent quantitative PCR assay for detection of Moniezia expansa mRNA with Moniezia expansa beta-Tubulin as an interna control to construct real-time fluorescent quantitative PCR assay.【Result】The results showed a good linear relationships(>0.991) between the Ct value and the concentration of positive plasmid for each gene.The melting curve analysis showed the product was specific to a single peak,with high specificity.【Conclusion】Real-time PCR results showed that the expression abundance of Annexins B3 gene was from high to low in gravid segment,scolex,mature segment and immature segment in order. |
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| Keywords: | Annexins B3 Moniezia expansa RT-PCR differential expression |
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