| 谷氨酸棒状杆菌gdh基因在黄色短杆菌C-11中的克隆及表达 |
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| 引用本文： | 王宁,包慧芳,杨慧,王炜.谷氨酸棒状杆菌gdh基因在黄色短杆菌C-11中的克隆及表达[J].新疆农业科学,2011(9):1711-1715. |
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| 作者姓名： | 王宁 包慧芳 杨慧 王炜 |
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| 作者单位： | 新疆农业科学院微生物应用研究所; |
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| 基金项目： | 新疆维吾尔自治区高技术研究发展计划(200910102); 新疆维吾尔自治区重大专项(200740147-2); 新疆维吾尔自治区科技支疆项目(201091227); 新疆维吾尔自治区科技基础条件平台建设项目(PT0807) |
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| 摘 要： | 【目的】研究谷氨酸脱氢酶的表达对L-丝氨酸产率及发酵效率的影响。【方法】从谷氨酸棒杆菌模式菌株C.glutamicum ATCC39135中克隆出gdh基因,以大肠杆菌-黄色短杆菌穿梭表达载体pEC7为基础,构建重组质粒pEC7G,并转化黄色短杆菌B.flavum C-11,获得重组菌株C-11G。【结果】重组菌株C-11G的GDH酶活力提高32.7%,产酸率为15.28 g/L,比原宿主菌提高了33.80%。【结论】成功构建重组菌株C-11G,所克隆的gdh基因有较高表达活性,并对L-丝氨酸产量的提高有促进作用。
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| 关 键 词： | 谷氨酸脱氢酶 pEC7 穿梭表达 电击转化 |
The Cloning and Expression of gdh Gene from Corynebacterium glutamicum in Brevibacterium flavum C-11 |
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| WANG Ning,BAO Hui -Fang,WANG Wei.The Cloning and Expression of gdh Gene from Corynebacterium glutamicum in Brevibacterium flavum C-11[J].Xinjiang Agricultural Sciences,2011(9):1711-1715. |
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| Authors: | WANG Ning BAO Hui -Fang WANG Wei |
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| Institution: | WANG Ning,BAO Hui -Fang,WANG Wei (Research Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China ) |
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| Abstract: | Objective]The purpose of this project was to study L - serine production rate and the fermentation efficiency affected by the expression of GDH.Method]The gdh(glutamate dehydrogenase) gene was cloned from Corynebacterium glutamicum ATCC39135 and amplified by PCR.The gdh gene was inserted into E.coli - Brevibacterium flavum shuttle expression vector pEC7 to construct a recombinant plasmid pEC7G, which was subsequently transformed into B.Jlavum C - 11,producing a genetic engineering strain C - 11G. Result]... |
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| Keywords: | glutamate dehydrogenase pEC7 shuttle expression electroporation |
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