不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

【目的】研究新疆不同地理种群棉蚜3种酶的活性及抗药性的差异。【方法】测定新疆库尔勒、五家渠、石河子、奎屯、哈密地区棉蚜和实验室敏感种群羧酸酯酶、谷胱甘肽-S-转移酶、乙酰胆碱酯酶活性,并分析3种酶活性与吡虫啉抗性之间的关系。【结果】库尔勒、五家渠、石河子、奎屯和哈密地区乙酰胆碱酯酶和谷胱甘肽-S-转移酶的比活力比值分别为4.2、2.7、2.3、2.5、2.1倍和2.3、1.5、1.3、2.5、2.1倍,差异显著（P<0.05）;羧酸酯酶比活力比值为6.5、4.2、3.4、2.7、1.2倍,除哈密地区相对接近外,其余地区均明显高于1.0,差异显著（P<0.05）,2种棉蚜关键解毒酶和1种神经传导关键性酶与抗药性之间存在一定相关性。【结论】各地理种群棉蚜3种酶活性均显著高于敏感种群（P<0.05）,且彼此之间存在差异性,羧酸酯酶在棉蚜感受到吡虫啉杀虫剂胁迫时极有可能起到重要作用。

Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

【Objective】Objective to explore the difference of three enzyme activities and drug resistance of different geographical populations of Aphis gossypii in Xinjiang. 【Methods】 The activities of carboxylesterase, glutathione-S-transferase and acetylcholinesterase of Aphis gossypii and laboratory sensitive populations in Korla, Wujiaqu, Shihezi, Kuitun and Hami of Xinjiang were determined, and the relationship between the activities of the three enzymes and imidacloprid resistance was analyzed. 【Results】 The specific activity ratios of acetylcholinesterase and glutathione-S-transferase in Korla, Wujiaqu, Shihezi, Kuitun and Hami were 4.2, 2.7, 2.3, 2.5, 2.1 times and 2.3, 1.5, 1.3, 2.5, 2.1 times, respectively（P < 0.05）; the specific activity ratios of carboxylesterase were 6.5, 4.2, 3.4, 2.7, 1.2 times, except Hami, the other areas were significantly higher than 1.2 times There was a certain correlation between two key detoxification enzymes and one key neurotransmitter enzyme of Aphis gossypii and drug resistance.【Conclusion】 The results showed that the activities of three kinds of enzymes in different populations were significantly higher than those in sensitive populations（P <0.05）, and there were differences among them. Carboxylesterase may play an important role in the process of Aphis gossypii feeling imidacloprid stress.