伊犁马LPL基因多态性及其与肉质性状关联性分析

【目的】研究伊犁马脂蛋白脂酶(lipoprotein lipase,LPL)基因多态性及其对肉质性状的影响,为专门化肉用马选种提供技术支撑。【方法】以41匹成年伊犁马为研究对象,采集颈静脉血液样本及其三角肌、背阔肌、臂三头肌、腹外斜肌和臀中肌5个部位肌肉样本,提取伊犁马DNA样本并测定肉质性状,利用PCR测序法筛选LPL基因突变位点并进行多态性分析,结合伊犁马肉质性状数据与LPL基因多态性进行关联性分析。【结果】所选马匹中共检测出2个突变位点(g.715T>C和g.12074C>A),均为内含子突变,2个位点均只检测出2种基因型,为低度多态位点。g.715T>C位点TT基因型马匹背阔肌和臀中肌失水率显著高于TC基因型马匹(P<0.05),背阔肌剪切力极显著大于TC基因型马匹(P<0.01),腹外斜肌剪切力极显著小于TC基因型马匹(P<0.05),三角肌和臀中肌剪切力显著大于TC基因型马匹(P<0.05);g.12074C>A突变位点CC基因型马匹臂三头肌和臀中肌失水率极显著低于CA基因型马匹(P<0.01),背阔肌和腹外斜肌失水率显著低于CA基因型马匹(P<0.05),臂三头肌熟肉率极显著高于CA基因型马匹(P<0.01),三角肌和腹外斜肌熟肉率与CA基因型马匹呈显著差异(P<0.05),臂三头肌和腹外斜肌剪切力极显著高于CA基因型马匹(P<0.01)。【结论】LPL基因g.715T>C和g.12074C>A位点是影响伊犁马肉质性状的潜在位点。

Polymorphism of LPL Gene and Its Relationship with Meat Quality Traits in Yili Horse

【Objective】 To investigate the polymorphism of lipoprotein lipase gene (LPL)and its effect on meat quality traits in Yili horses, and to provide technical support for the selection of specialized meat horses. 【Methods】 In this study, blood samples were collected from 41 adult Yili horses from the jugular vein and five muscle parts: deltoid muscle, latissimus dorsi, arm triceps, external oblique muscle and gluteal muscle to extract Yili horse DNA samples and determine meat quality traits. PCR sequencing was used to screen LPL gene mutation loci and perform polymorphism analysis, combined with data on meat quality traits of Yili horse and LPL gene polymorphism for association analysis. 【Results】 Two mutant loci (g.715T>C and g.12074C>A) were detected in the selected horses, both of which were intronic mutations. Only 2 genotypes were detected for both loci, which were low polymorphic loci. g.715T>C locus TT genotype horses had significantly higher dorsalis latissimus and gluteal musclewater loss rate than TC genotype horses(P<0.05), significantly greater dorsalis latissimus shear force than TC genotype horses(P<0.01), significantly less external oblique muscleshear force than TC genotype horses(P<0.05), and significantly greater deltoid muscle and gluteal muscleshear force than TC genotype horses(P<0.05). g. 12074C > A mutant horses with the CC genotype had a significantly lower water loss rate in the arm triceps and gluteal muscle(P<0.01), a significantly lower water loss rate in the latissimus dorsi and oblique abdominis than in the CA genotype(P<0.05), a significantly higher cooked meat rate in the arm triceps than in the CA genotype(P<0.01), a significantly difference of cooked meat rate was found in the deltoid muscle and oblique abdominis than in the CA genotype(P<0.05), and a significantly higher rate of shear force in the arm triceps and oblique abdominis than in the CA genotype(P<0.01).【Conclusion】 The g.715T>C and g.12074C>A loci of the LPL gene may be potential loci for meat quality traits in Yili horses.