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| 黔北一新鱼种遵义小钝吻鮠线粒体DNA的分离纯化 | |
| 引用本文： | 肖贵榜,葛正龙,张艳磊,李作祥.黔北一新鱼种遵义小钝吻鮠线粒体DNA的分离纯化[J].现代农业科技,2011(19):325-326. |
| 作者姓名： | 肖贵榜 葛正龙 张艳磊 李作祥 |
| 作者单位： | 1. 遵义职业技术学院,贵州遵义,563000 2. 遵义医学院生物化学与分子生物学教研室 3. 贵阳职业技术学院生物化学工程系 |
| 摘 要： | 用差速离心法和改进的碱裂解法分离纯化遵义小钝吻鮠线粒体DNA,紫外检测其OD260/OD280在1.78～1.85,并计算出该鱼肝组织线粒体DNA的含量为0.613μg/g。纯化样品经紫外分析仪在200～290 nm波长范围内扫描,呈现典型的DNA吸收峰,其最大吸收峰在259～260 nm。以λDNA/HindⅢ的完全酶切片段作为相对分子量标准,利用电泳迁移率与分子量的对数的线性关系,由已知片段大小推算出遵义小钝吻鮠mtDNA分子大小为(15.44±0.16)kb。 |
| 关 键 词： | 遵义小钝吻鮠 线粒体DNA 分离纯化 黔北地区 |
Isolation and Purification of Mifochondrial DNA of New Species of Leioxassis Bleeker in Northern Guizhou |
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| Authors: | XIAO Gui-bang GE Zheng-long ZHANG Yan-lei LI Zuo-xiang |
| Institution: | XIAO Gui-bang 1,2 GE Zheng-long 2 ZHANG Yan-lei 2 LI Zuo-xiang 3(1 Zunyi Vocational and Technical College,Zunyi Guizhou 563000,2 Department of Biochemistry,Zunyi Medical College,3 Department of Biochemical Engineering,Guiyang Vocational and Technical College) |
| Abstract: | The mitochondrial DNA（mtDNA）from liver ofLeioxassis~ticrocrassirostris ZunyiensisXiao sp.nov.was isolated and purified by means of differential centrifugation and the improved alkaline lysisoThe OD260/OD280 was 1.78-1.85 by UV spectrophotometer,and the quality of mtDNA obtained was 0.613 μg/goUnder the wavelength scan of 200-290 nm by UV solution ,the Purification of DNA of the sample presented characteristic DNA absorption peaks and the maximum one in 259-260 nmoUsekDNA/Hind m restriction fragments as a relatively molecular weight standard, according to the electrephoretic migration rate was linearly related to the molecular weight, the mtDNA size was （15.44±0.16） kb in the fish . |
| Keywords: | Leioxassis Bleeker mtDNA solation purification northern Guizhou area |
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