| 远志茎愈伤组织无性系建立的研究 |
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| 引用本文： | 赵鑫鑫,于洋,廖萍,宁淑香,姜长阳.远志茎愈伤组织无性系建立的研究[J].现代农业科技,2011(17):182-183. |
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| 作者姓名： | 赵鑫鑫 于洋 廖萍 宁淑香 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目(20090304); 辽宁师范大学教学改革资助项目(LSJG:20090108) |
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| 摘 要： | 为保护野生资源,实现人工栽培,以远志的叶片、嫩茎和嫩根为材料,采用组织培养的方法,进行愈伤组织的诱导和分化,试管苗的生根、移栽和定植的研究,建立远志嫩根无性系。结果表明:MS+BA 0.5 mg/L+2,4-D 1.5 mg/L+NAA 0.5 mg/L是嫩根愈伤组织诱导培养和继代培养的理想培养基;MS+BA 0.8 mg/L+NAA 0.1 mg/L+AgNO3 1.8 mg/L和MS+BA 0.8 mg/L+NAA 0.1 mg/L+AgNO3 2.1 mg/L 2种培养基是嫩根愈伤组织分化培养的理想培养基;1/4 MS+NAA 0.2 mg/L是不定芽生根培养和试管苗生根继代培养的理想培养基;定植成活的试管苗保持了野生远志的所有生物学性状。
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| 关 键 词： | 远志 愈伤组织 无性系 |
Study on Establishment of the Clone of Polygala Tennuifolia Callus |
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| Authors: | ZHAO Xin-xin YU Yang LIAO Ping NING Shu-xiang JIANG Chang-yang |
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| Institution: | ZHAO Xin-xin YU Yang LIAO Ping NING Shu-xiang JIANG Chang-yang (College of Life Science,Liaoning Normal University,Dalian Liaoning 116029) |
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| Abstract: | In order to protect wild resources and realize artificial cultivation,the leaves,tender stems and roots of Polygala Tennuifolia were taken as material to investigate callus inducement and differentiation,rooting of tube seedlings and transplanting with the method of tissue culture,the clone of Polygala tennuifolia from tender roots was established.The results indicated that the ideal medium for callus induction and multiplication of tender roots was MS+BA 0.5 mg/L+2,4-D 1.5 mg/L+NAA 0.5 mg/L;MS+BA 0.8 mg/L+... |
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| Keywords: | Polygala tennuifolia callus clone |
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