|
|||||
|
|
| 烟草黑胫病菌拮抗细菌的筛选与鉴定 | |
| 引用本文： | 王栋,伏晓,韩新宇,罗定棋,何余勇,张永辉,雷晓.烟草黑胫病菌拮抗细菌的筛选与鉴定[J].现代农业科技,2012(16):133-134,141. |
| 作者姓名： | 王栋 伏晓 韩新宇 罗定棋 何余勇 张永辉 雷晓 |
| 作者单位： | 1. 四川省烟草公司泸州市公司,四川泸州,646000 2. 山东省烟草公司临沂市公司 3. 中国农业科学院烟草研究所 |
| 基金项目： | 泸州市烟草公司科技项目 |
| 摘 要： | 从烟草根际土壤分离到107个细菌分离物,通过室内平板对峙培养筛选,筛选到13株对烟草黑胫病菌有拮抗作用的菌株,其中,LZ-7菌株的室内平板抑菌效果最好,抑菌圈直径达27.82 mm。经盆栽试验,LZ-7、A3控病作用较好,防治效果分别达59.12%和55.84%。通过16 S rDNA序列测定与分析,LZ-7与Bacillus pumilus的同源性为99%,初步鉴定证明该菌株属于短小芽孢杆菌。 |
| 关 键 词： | 烟草黑胫病 拮抗细菌 生物防治 筛选 鉴定 |
Screening and Identification of Antagonistic Bacteria Against Phytophthora parasitica var. nicotianae |
|
| Authors: | WANG Dong FU Xiao HAN Xin-yu LUO Ding-qi HE Yu-yong ZHANG Yong-hui LEI Xiao |
| Institution: | 1(1 Luzhou Tobacco Company in Sichuan Province,Luzhou Sichuan 646000;2 Linyi Tobacco Company in Shandong Province; 3 Tobacco Research Institute of Chinese Academy of Agricultural Sciences) |
| Abstract: | Totally 107 bacterial strains were isolated from different tobacco rhizosphere soils,of which 13 strains showed antagonistic activity against phytophthora parasitica var.nicotianae.LZ-7 strain had the best room flat antibacterial activity and inhibition zone diameter reached 27.82 mm.Pot experiment showed that the LZ-7 and A3 had better control effect which reached 59.12% and 55.84%.The 16S rDNA fragment was amplified from strain LZ-7 by PCR,and the 16S rDNA sequence analysis indicated that strain LZ-7 shared 99% 16S rDNA sequence homology with Bacillus pumilus.In the phylogenetic framework of bacterial classification,LZ-7 belonged to Bacillus pumilus. |
| Keywords: | Phytophthora parasitica var nicotianae antagonistic bacteria biological control screening identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |