| 辅酶Q10产生菌的筛选及产物的定量检测 |
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| 引用本文： | 陈锐,孙晓宇,韩丽萍,沈俭,门欣,路鹏鹏,沈卫荣.辅酶Q10产生菌的筛选及产物的定量检测[J].现代农业科技,2012(16):289-290,296. |
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| 作者姓名： | 陈锐 孙晓宇 韩丽萍 沈俭 门欣 路鹏鹏 沈卫荣 |
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| 作者单位： | 陕西省微生物研究所,陕西西安,710043 |
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| 基金项目： | 陕西省科学院青年人才培养基金项目 |
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| 摘 要： | 为了从实验室保存的酵母菌株中筛选产生辅酶Q10的菌株,采用麦芽汁培养酵母菌,皂化法萃取菌体中的辅酶Q10,可见光比色法和高压液相色谱法测定酵母菌中的辅酶Q10的含量。结果表明:筛选出2株较高产量的辅酶Q10产生菌,热带假丝酵母(C.tropicalis(cast).Berkhout)B021产量为7.420 mg/L、粟酒酵母(Schizosaccheromyces pombe Lindner)B147产量为7.488 mg/L。
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| 关 键 词： | 辅酶Q10 筛选 菌株 |
Research on Screening of Coenzyme Q10 Production Strain and Coenzyme Q10 Quantification |
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| Authors: | CHEN Rui SUN Xiao-yu HAN Li-ping SHEN Jian MEN Xin LU Peng-peng SHEN Wei-rong |
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| Institution: | *(Microbiology Institute of Shaanxi Province,Xi’an Shaanxi 710043) |
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| Abstract: | In order to Screen out coenzyme Q10 production strain from the saved yeast strains,the paper cultured yeast strain with wort medium and extracted coenzyme Q10 with the alkaline alcohol saponification processes,and then spectrophotometer and HPLC were used to analyze the quantificate of coenzyme Q10 in this thesis.Results showed that two strain could express coenzyme Q10:C.tropicalis(cast).Berkhout B021(7.420 mg/L),Schizosaccheromyces pombe Lindner B147(7.488 mg/L),respectively. |
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| Keywords: | Coenzyme Q10 screening strain |
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