大三叶升麻嫩茎无性系的建立

为保护野生资源,实现人工栽培,以大三叶升麻茎为试验材料,进行愈伤组织的培养,以及试管苗的生根、生根继代培养和试管苗的移栽、定植的研究,建立起野生大三叶升麻无性系。结果表明:MS+BA 0.2 mg/L+2,4-D 2.0～2.5 mg/L是嫩茎愈伤组织诱导培养的理想培养基;MS+AgNO31.0 mg/L+GA30.5 mg/L+ZT 0.8 mg/L+NAA 0.1 mg/L是嫩茎愈伤组织分化培养的理想培养基;把不定芽的基部在NAA 4.0mg/L的溶液中处理约5 min,接种到1/3MS为基本培养基,附加IBA 0.6 mg/L的培养基上,进行生根培养的方法是生根培养的理想培养方法;1/3MS+IBA 0.6 mg/L+NAA 0.1 mg/L也是增殖培养的理想培养基;试管苗移栽、定植易成活。定植成活的试管苗保持了野生大三叶升麻的所有植物学性状。

Establishment of Tender Stem Clone for Cimicifuga Simpiex

For the protection of the wild resources and realization artificial cultivation,the tender stems of Cimicifuga simpiex were used as materials to do the study by using tissue culture methods.The study was mainly about callus induction and differentiation,adventitious buds rooting and rooting of the subculture,tube seedlings transplanting colonization,and establishment the clone of Cimicifuga simpiex.The results showed that MS+BA 0.2 mg/L+2,4-D 2.0～2.5 mg/L and MS+AgNO3 1.0 mg/L+GA3 0.5 mg/L+ZT 0.8 mg/L+NAA 0.1 mg/L were the optimum media for callus induction and differentiation respectively.After dealt with 4.0 mg/L NAA for 5 minutes,the adventitious buds could be induced rooting in the medium of 1/3MS+IBA 0.6 mg/L.The ideal medium for rooting of the subculture was 1/3MS+IBA 0.6 mg/L+NAA 0.1 mg/L.The tube seedlings could be survived after transplanting colonization.The colonization of plantlets maintained all biological traits which wild plant has.