彩叶草组织培养与快速繁殖技术研究

以彩叶草节段为外植体进行离体培养,对外植体灭菌方法以及培养基中不同种类外源激素的浓度对试管苗增殖、生根的影响等进行了研究。结果表明:以0.1%HgCl2灭菌8 min,无菌外植体成活率可以达到70%;在MS+6-BA 2.0 mg/L+NAA 0.20 mg/L的增殖培养基上培养30 d,试管苗的增殖系数可达8.8;在1/2MS+6-BA 0.2 mg/L+NAA 0.5 mg/L的生根培养基上培养21 d后,生根率为100.0%,生根5.6条/株;炼苗后移植成活率达95%。

Research on Tissue Culture and Rapid Propagation of Coleus blumei

The in vitro culture of Coleus blumei was established by using the nodal stem segments as explants.The method of surface sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting were investigated.The results showed that 70% sterile explants could be obtained by surface treatment with 0.1% HgCl2for 8 minutes.When cultured on a MS multiplication medium supplemented with 2.0 mg/L of 6-BA and 0.2 mg/L of NAA for 30 d,the test-tube seedling exhibited a multiplication coefficient of 8.8.For optimal rooting,induction on a 1/2 MS rooting medium supplemented with 0.2 mg/L of 6-BA and 0.5 mg/L of NAA for 21 days could produce rooted plantlets and each plantlet generated 5.6 roots on average.Rooted plantlets were acclimatized and transferred into outdoor environment with 95% survival rate.