| 乙醇浸提法提取黑穗醋栗果实中黄酮类物质的研究 |
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| 引用本文： | 边啸林,李政,王广正,徐雅琴.乙醇浸提法提取黑穗醋栗果实中黄酮类物质的研究[J].现代农业科技,2009(11):24-25. |
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| 作者姓名： | 边啸林 李政 王广正 徐雅琴 |
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| 作者单位： | 东北农业大学理学院,黑龙江哈尔滨,150030 |
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| 基金项目： | 东北农业大学大学生创新课题基金(20072006) |
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| 摘 要： | 以黑穗醋栗果实为试材，以芦丁为标准品，利用分光光度法研究乙醇提取法提取黄酮类物质的最佳工艺条件。在单因素试验结果的基础上设计了h（34）（4因素3水平）正交试验。结果表明，乙醇提取法提取黄酮的最佳工艺条件为：以95％乙醇为提取剂，提取温度90℃，提取料液比1：12。提取时间180min。黄酮的最大提取量可达0．723mg／g。
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| 关 键 词： | 黑穗醋栗 黄酮 乙醇浸提 提取 |
Study on Ethanol Extraction of Flavonoids from Blackcurrant |
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| Authors: | BIAN Xiao-lin LI Zheng WANG Guang-zheng XU Ya-qin |
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| Institution: | College of Science;Northeast Agricultural University;Harbin Heilongjiang 150030 |
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| Abstract: | The contents of total flavonoids in black currant fruit were determined by spectrophotometric methods in terms of rutin equivalent.First of all,the single factor experiments were conducted,then orthogonal experiment of L9(34)by colorimetric estimation was adopted.The results showed that the optimum process of extraction was obtained,when 95% ethanol was seleted as extraction agent and extracting ratio was 1∶12 under 90℃ for 180 minutes.Extraction capacity was 0.723mg/g at most. |
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| Keywords: | black currant flavonoids ethanol extraction |
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