| 进境百合种球上3种线虫的检疫鉴定研究 |
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| 引用本文： | 杨小凤,祝秀丽,陆辰晨,邵沛泽,谌运清,赵光明,杨万风,汪连军,潘杰.进境百合种球上3种线虫的检疫鉴定研究[J].现代农业科技,2022(6). |
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| 作者姓名： | 杨小凤 祝秀丽 陆辰晨 邵沛泽 谌运清 赵光明 杨万风 汪连军 潘杰 |
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| 作者单位： | 连云港海关,连云港海关,连云港海关,连云港海关,连云港海关,连云港海关,连云港海关,连云港海关,连云港海关 |
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| 摘 要： | 本文通过改良贝曼漏斗法分离收集进境荷兰百合种球及介质土中携带的线虫，其中3种线虫种类经形态特征观察、显微测计及核糖体DNA 28S-D2/D3区序列分析，分别鉴定为洞穴长尾线虫（Seinura caverna）、毁芽滑刃线虫（Aphelenchoides blastophthorus）和燕麦真滑刃线虫（Aphelenchus avenae），均为江苏口岸首次截获，目前，洞穴长尾线虫（S.caverna）、毁芽滑刃线虫（A.blastophthorus）国内现有报道较少。本文主要通过对江苏口岸首次截获的3种线虫进行检疫鉴定研究，以期为口岸进境花卉种球检疫监管提供参考。
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| 关 键 词： | 百合种球 洞穴长尾线虫 毁芽滑刃线虫 燕麦真滑刃线虫 形态特征 检疫监管 |
| 收稿时间： | 2021/6/30 0:00:00 |
| 修稿时间： | 2021/6/30 0:00:00 |
Quarantine and identification of three nematodes intercepted from imported lily bulbs |
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| Abstract: | In this paper, the nematodes from the imported lily bulbs and the medium soil were extracted using modified Baermann funnel method and identified based on morphological characteristics, morphometrics and sequence analysis of the 28S rDNA-D2/D3. Three species of nematodes were identified as Seinura caverna, Aphelenchoides blastophthorus and Aphelenchus avenae, which were intercepted for the first time at Jiangsu port. At present, there are few reports for S.caverna and A. brastophthorus in China. In this paper, three species of nematodes intercepted for the first time at Jiangsu port were studied for quarantine identification in order to provide references for quarantine supervision of imported flower bulbs. |
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