| 高产单细胞蛋白酵母的诱变育种及培养条件优化 |
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| 引用本文： | 王程,霍冀川,刘佳琪,袁艳红.高产单细胞蛋白酵母的诱变育种及培养条件优化[J].现代农业科技,2010(9):16-18,21. |
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| 作者姓名： | 王程 霍冀川 刘佳琪 袁艳红 |
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| 作者单位： | 西南科技大学应用化学研究所,四川绵阳,621010 |
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| 基金项目： | 四川省应用基础研究计划 |
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| 摘 要： | 从啤酒废酵母泥中分离纯化出5株酿酒酵母,以其中单细胞蛋白(SCP)含量较高的SY-5为出发菌株,通过紫外诱变分生孢子,经过初筛和复筛,得到1株高产SCP的诱变菌株SY-5-7,比出发菌株高7.84%,达到48.67%。通过单因素试验对诱变菌株SY-5-7培养条件进行优化,结果表明,最适培养条件为培养基起始pH值5.0、培养温度26℃、接种量2%、发酵周期为70h,碳源为50g/L蔗糖、氮源为10g/L酵母膏。在此基础上进行培养,SY-5-7的生物量比初始条件提高了约35%。
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| 关 键 词： | 酿酒酵母 单细胞蛋白 紫外诱变 培养条件 |
Screening of Yeast with High-yield Single Cell Protein by UV Mutation anti Optimization of its Culture Conditions |
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| Authors: | WANG Cheng HUO Ji-chuan LIU Jia-qi YUAN Yan-hong |
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| Institution: | WANG Cheng HUO Ji-chuan LIU Jia-qi YUAN Yan-hong (Institute of applied chemistry,Southwest University of Science and Technology,Mianyang 621010) |
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| Abstract: | Five strains of Saccharomyces cerevisiae are isolated from waste brewer yeast,SY-5 which had a higher productivity of SCP was selected as the starting strain for stepwise mutation by UV irrigation. After primary and secondary screening,a high-yield SCP strain named SY-5-7 was obtained from its parent strain. The percentage of SCP yield of SY-5-7 was 48.67%,increased by 7.84% compared with the original strain. Then the cultivating condition of the mutant strain was optimized by single-factor test. It was fou... |
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| Keywords: | Saccharomyces cerevisiae single cell protein UV irrigation cultivating condition |
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