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耐盐基因Hal1的克隆及表达载体的构建
引用本文:沈雁,陈霞,陈燕,龚毅,陈宇光.耐盐基因Hal1的克隆及表达载体的构建[J].现代农业科技,2009(7):243-244.
作者姓名:沈雁  陈霞  陈燕  龚毅  陈宇光
作者单位:[1]中科院上海生命科学研究院,上海200233 [2]上海大学生命科学院,上海200233
摘    要:以酵母菌株BWG1-7A基因组DNA为模板,利用PCR方法,扩增出耐盐基因Hal1,测序表明该基因全长为885个核苷酸,与已发表的序列NC_001148比较,同源性99.3%。将该基因插入表达载体pYES2的BamHI和EcoRI酶切位点之间。构建表达载体pYES2-Hal1,序列测定完全正确,为植物表达载体的构建打下基础。

关 键 词:耐盐性  Hal1基因  克隆

Cloning of Hal1 gene and construction of its expression plasmid
Authors:SHEN Yan  CHEN Xia CHEN Yan GONG Yi CHEN Yu-guang
Institution:1Research Center of Biotechnology Shanghai Institutes for Biological Sciences the chinese Academy of Sciences;Shanghai 200233;2School of life Sciences;Shanghai University
Abstract:Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template,Hal1 gene is amplified by PCR.The amplified product was digested with BamHI and EcoRI enzymes,then ligated into pYES2 vector.The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector.
Keywords:Salt tolerance  Hal1 gene  Cloning  
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