| 木聚糖酶基因xynA在大肠杆菌中的高效表达 |
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| 引用本文： | 殷尔康.木聚糖酶基因xynA在大肠杆菌中的高效表达[J].现代农业科技,2011(12):326-327. |
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| 作者姓名： | 殷尔康 |
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| 作者单位： | 江苏畜牧兽医职业技术学院食品科技系,江苏泰州,225300 |
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| 摘 要： | 参照Genebank中疏棉状嗜热丝孢菌木聚糖酶A基因序列,以引物拼接方式,人工合成采用大肠杆菌优势密码子的基因xynA,插入pET-20b载体,获得重组表达载体pET-20b-xynA。重组菌在16℃下诱导效果最好,10 h酶活达到42.05 U/mL,重组酶占全细胞蛋白的47%,粗酶在65℃和pH 5～8条件下很稳定。
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| 关 键 词： | 木聚糖酶A基因 优势密码子 高效表达 |
High-level Expression of Xylanase A in Escherichia coli |
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| Authors: | YIN Er-kang |
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| Institution: | YIN Er-kang(Food Technology Faculty of Jiangsu Animal Husbandry & Veterinary College,Taizhou Jiangsu 225300) |
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| Abstract: | According to the gene sequence in Genebank,a codon-optimized xylanase A gene(xynA)from Thermomyces lanuginosus was syn-thesized to construct the expression vector pET-20b-xynA.16 ℃ was the better induction temperature compared with 37 ℃.A maximum activity of 42.05 U/mL was detected after 10 hours of induction,and the expression level of recombinant xylanase was improved to 47%.The rude enzyme was stable at 65 ℃ and over the pH range of 5.0～8.0. |
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| Keywords: | xylanase A gene(xynA) preferred codons high-level expression |
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