草莓种子实生苗的病毒分子检测

草莓易感染病毒,从而导致产量减少和品质下降。目前生产上主要采用茎尖组织培养脱毒的方法,并不能保证完全脱除病毒,还需要进行无病毒检测鉴定。因此,需进一步探索简便、高效、免检的脱毒体系。利用RT-PCR技术,以草莓肌动蛋白基因序列为内标,结合4种草莓病毒特异性扩增,验证了草莓种子实生苗不携带病毒。旨在助推种子繁殖型草莓品种的应用。

Molecular Detection of Virus in Seedlings from Strawberry Seed

Strawberry can be infected by virus easily, which lead to yield and quality losses. At present, the method of stem tip tissue culture and detoxification is mainly used in production, which can not guarantee the complete removal of virus, but also needs to be detected and identified without virus. Therefore, it is necessary to further explore a simple, efficient and free-test detoxification system. By using the RT-PCR technique, based on strawberry actin gene as the internal control, and the specific amplification of 4 kinds of strawberry virus was combined to verify that the strawberry seed seedlings did not carry the virus. The aim is to promote the application of seed reproductive strawberry varieties.