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尾巨桉提纯复壮初步研究
引用本文:吴建宇,林星,蔡益航,邱金海,陈丽芳.尾巨桉提纯复壮初步研究[J].现代农业科技,2010(4):234-235.
作者姓名:吴建宇  林星  蔡益航  邱金海  陈丽芳
作者单位:1. 福建省莆田市生物工程研究所,福建莆田,351100
2. 福州市药品检验所
摘    要:以尾巨桉DH32-29最低位的萌芽条为外植体,利用组织培养进行继代重复培养(不少于5次),对比分析尾巨桉提纯复壮效果。结果表明:复壮苗与经过继代3年的未复壮苗相比,其继代周期缩短6d,增殖倍数提高1.09倍,生根率提高17.31%,移栽成活率提高20.19%,较未复壮苗有一定优势。

关 键 词:尾巨桉  提纯复壮  组织培养

Studies on Purification and Rejuvenescence of E.urophylla×E.grandis
Authors:WU Jian-yu  LIN Xing  CAI Yi-hang  QIU Jin-hai  CHEN Li-fang
Institution:1 Putian Institute of Bio-engineering in Fujian Province;Putian Fujian 351100;2 Fuzhou Medicinal Check Institute
Abstract:Based on tissue culture,taking bud tips from the lowest part of DH32-29(E.urophylla×E.grandis),subulture was taken at least five times aimed at comparing the effect of purification and rejuvenescence.Results showed that compared with the not rejuvenated material having the subculture cycle of 3 years,the rejuvenated material shorted 6 days,multiplication improved 1.09 times,rooting rate increased by 17.31%,and engraftment increased by 20.19%,thus it was superior to the not rejuvenated one.
Keywords:E  urophylla×E  grandis  purification and rejuvenescence  tissue culture
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