尾巨桉提纯复壮初步研究 |
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引用本文: | 吴建宇,林星,蔡益航,邱金海,陈丽芳.尾巨桉提纯复壮初步研究[J].现代农业科技,2010(4):234-235. |
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作者姓名: | 吴建宇 林星 蔡益航 邱金海 陈丽芳 |
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作者单位: | 1. 福建省莆田市生物工程研究所,福建莆田,351100 2. 福州市药品检验所 |
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摘 要: | 以尾巨桉DH32-29最低位的萌芽条为外植体,利用组织培养进行继代重复培养(不少于5次),对比分析尾巨桉提纯复壮效果。结果表明:复壮苗与经过继代3年的未复壮苗相比,其继代周期缩短6d,增殖倍数提高1.09倍,生根率提高17.31%,移栽成活率提高20.19%,较未复壮苗有一定优势。
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关 键 词: | 尾巨桉 提纯复壮 组织培养 |
Studies on Purification and Rejuvenescence of E.urophylla×E.grandis |
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Authors: | WU Jian-yu LIN Xing CAI Yi-hang QIU Jin-hai CHEN Li-fang |
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Institution: | 1 Putian Institute of Bio-engineering in Fujian Province;Putian Fujian 351100;2 Fuzhou Medicinal Check Institute |
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Abstract: | Based on tissue culture,taking bud tips from the lowest part of DH32-29(E.urophylla×E.grandis),subulture was taken at least five times aimed at comparing the effect of purification and rejuvenescence.Results showed that compared with the not rejuvenated material having the subculture cycle of 3 years,the rejuvenated material shorted 6 days,multiplication improved 1.09 times,rooting rate increased by 17.31%,and engraftment increased by 20.19%,thus it was superior to the not rejuvenated one. |
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Keywords: | E urophylla×E grandis purification and rejuvenescence tissue culture |
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