| 苜蓿多糖提取及测定方法的比较 |
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| 引用本文： | 赵大云,杨丽娥,赵仪华,胡雪华.苜蓿多糖提取及测定方法的比较[J].上海交通大学学报(农业科学版),2004,22(3):256-261. |
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| 作者姓名： | 赵大云 杨丽娥 赵仪华 胡雪华 |
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| 作者单位： | 上海交通大学,农业与生物学院,上海,201101 |
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| 基金项目： | 上海市科委发展基金资助项目(013912106) |
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| 摘 要： | 本文对水煮法和醇提法两种不同提取苜蓿多糖方法以及蒽酮-硫酸法和苯酚-硫酸法两种不同的苜蓿多糖测定方法进行了比较。结果发现，在进行苜蓿粗多糖的提取时，利用水煮法提取粗多糖可显著提高多糖得率。用蒽酮-硫酸法和苯酚-硫酸法两种不同的测定方法来测定多糖含量，在未脱蛋白质前，两种测定方法皆具有良好的稳定性、精密性以及重现性，但都受到样品中蛋白质干扰严重；而在脱蛋白后，蒽酮-硫酸法在检测样品不同稀释度的重现性稍优于苯酚-硫酸法。
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| 关 键 词： | 苜蓿多糖 提取 含量测定 水煮法 醇提法 蒽酮-硫酸法 苯酚-硫酸法 |
| 文章编号： | 1671-9964(2004)03-0256-05 |
| 修稿时间： | 2003年12月27 |
The Comparison of Extraction And Determination Methods Applied for Alfalfa Polysaccharides |
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| ZHAO Da-yun,YANG Li-e,ZHAO Yi-hu,HU Xue-hua.The Comparison of Extraction And Determination Methods Applied for Alfalfa Polysaccharides[J].Journal of Shanghai Jiaotong University (Agricultural Science),2004,22(3):256-261. |
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| Authors: | ZHAO Da-yun YANG Li-e ZHAO Yi-hu HU Xue-hua |
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| Abstract: | Two extraction methods and two assay methods were applied to analy si s polysaccharides in alfalfa. As a result, between the two extraction methods of boiling water-extraction and alcohol-precipitation to separate polysaccharide s from alfalfa, water-extraction method is more suitable than that of alcohol-precipitation. Between the two spectrophotometric methods of anthrone-sulfuric acid and phenol-sulfuric acid for polysaccharide determination, it was discover ed that both methods present same reproducibility before removing protein in the sample. But after removal of protein in the sample, the reproducibility of anth rone-sulfuric acid spectrophotometric method is better than that of phenol-sul furic acid spectrophotometric method. |
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| Keywords: | alfalfa alfalfa polysaccharides anthrone-sulfuric acid spectroph otometric method phenol-sulfuric acid spectrophotometric method |
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