胶体金免疫层析试纸结合RT-PCR法快速检测烟草环斑病毒

利用烟草环斑病毒胶体金免疫层析检测试纸条，对烟草环斑病毒3个分离物进行了快速检测。结果表明，试纸条在10min内，可特异性检出烟草环斑病毒的3个分离物，对番茄环斑病毒、南芥菜花叶病毒、番茄黑环病毒和健康叶片无反应。剪下试纸条上显示的检测带，进行RT—PCR，能扩增到与预期大小相同的DNA条带。试纸条检测烟草环斑病毒粗提纯液，检测灵敏度在1μg·mL^-1，试纸条检测烟草环斑病毒的病汁液，检测灵敏度在10^-3（W／V），试纸条检测灵敏度与DAS—ELISA方法灵敏度基本相同。

The Rapid Detection of Tobacco Ringspot Virus by Gold Immunochromatography Assay and RT-PCR

Three isolates of tobacco ringspot virus were rapidly detected with gold immunochromatography assay （GICA , Immunostrip）. The results indicated that the 3 isolates of TRSV could be tested specifically on immunostrip within 10 minutes and no-specific reaction was observed for the ToRSV, ArMV, TBRV and healthy leaves. RT-PCR amplification could produce a specific DNA band of the expected size with the reaction band cut on immunostrip. The sensitivity of the immunostrip was 1μg·mL^-1 for the partial purified viruses. Extract from TRSV- infected tobacco leaves was detected to a dilution of 10^-3（W/V）. The sensitivity of the immunostrip and DAS-ELISA was almost the same.