| 茄花青素5-O糖基转移酶基因克隆与表达特征分析 |
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| 引用本文： | 李翔,刘杨,李怀志,王中彦,陈火英.茄花青素5-O糖基转移酶基因克隆与表达特征分析[J].上海交通大学学报(农业科学版),2011,29(6):1-5,23. |
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| 作者姓名： | 李翔 刘杨 李怀志 王中彦 陈火英 |
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| 作者单位： | 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2006)第2-1号]; 上海市重点学科建设项目(B209) |
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| 摘 要： | 以云南长紫茄果皮为实验材料,采用同源克隆技术和RACE结合的方法,克隆到茄子花青素5-O糖基转移酶基因(5GT).该基因编码区为1 413 bp,与矮牵牛5GT基因序列同源性为87.4％.花青素含量测定和半定量PCR结果表明,该基因在云南紫长茄的花瓣和成熟果皮中的表达量要高于云南圆白茄；而在叶片和未成熟果皮中,2个品种...
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| 关 键 词： | 茄子 花青素 花青素5-O糖基转移酶 表达分析 |
Cloning and Expression Characterization of the Anthocyanin 5-O Glucosyltransferase in Eggplant |
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| LI Xiang,LIU Yang,LI Huai-zhi,WANG Zhong-yan,CHEN Huo-ying.Cloning and Expression Characterization of the Anthocyanin 5-O Glucosyltransferase in Eggplant[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(6):1-5,23. |
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| Authors: | LI Xiang LIU Yang LI Huai-zhi WANG Zhong-yan CHEN Huo-ying |
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| Institution: | LI Xiang,LIU Yang,LI Huai-zhi,WANG Zhong-yan,CHEN Huo-ying (School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Using homologous cloning and rapid amplification of cDNA ends(RACE),a cDNA fragment of 5GT(UDP-glucose;anthocyanin 5-O gIucosyItransferase)gene was cloned from the eggplant cultivar,Yunnan long-purple eggplant.This cDNA fragment includes 1 413 bp;Its identity with the Petunia x hybrida 5GT was 87.4%,Using Yunnan long-purple eggplant and Yunnan round-white eggplant as materials,semi-quantitative RT-PCR and anthocyanin content tests were conducted to characterize the expression feature of 5GT gene.The express... |
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| Keywords: | eggplant anthocyanin anthocyanin 5-O glucosyltransferase gene cloning expression analysis |
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