| 短小芽孢杆菌(Bacillus pumilus)DX01转座突变株的构建及转化体系的优化 |
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| 引用本文： | 胡晓璐,沈新迁,傅科鹤,顾振芳,陈云鹏. 短小芽孢杆菌(Bacillus pumilus)DX01转座突变株的构建及转化体系的优化[J]. 上海交通大学学报(农业科学版), 2011, 29(1): 68-74. DOI: 10.3969/J.ISSN.1671-9964.2011.01.013 |
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| 作者姓名： | 胡晓璐 沈新迁 傅科鹤 顾振芳 陈云鹏 |
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| 作者单位： | 上海交通大学,农业与生物学院,上海,200240 |
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| 基金项目： | 国家自然科学基金资助课题(31071721); 上海市白玉兰科技人才基金(2010B046); 农业部都市农业(南方)重点实验室开放课题(09UA005) |
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| 摘 要： | Tn5转座子及其衍生载体被广泛应用于革兰氏阴性菌的基因功能研究,但尚未见有利用Tn5转座子导入革兰氏阳性菌短小芽孢杆菌(Bacillus pumilus)DX01菌株的报道.本研究通过构建Tn5转座载体,对短小芽孢杆菌DX01菌株进行了Tn5转座插入诱变,获得了大量的突变株.同时,考察了感受态细胞培养浓度、电击电压、电...
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| 关 键 词： | 短小芽孢杆菌 突变 Tn5转座子 电击转化 |
Generation of Bacillus pumilus DX01 Mutations with Tn5 Transposon and Optimization of Its Electrotransformation System |
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| HU Xiao-lu,SHEN Xin-qian,FU Ke-he,GU Zhen-fang,CHEN Yun-peng. Generation of Bacillus pumilus DX01 Mutations with Tn5 Transposon and Optimization of Its Electrotransformation System[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2011, 29(1): 68-74. DOI: 10.3969/J.ISSN.1671-9964.2011.01.013 |
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| Authors: | HU Xiao-lu SHEN Xin-qian FU Ke-he GU Zhen-fang CHEN Yun-peng |
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| Affiliation: | HU Xiao-lu,SHEN Xin-qian,FU Ke-he,GU Zhen-fang,CHEN Yun-peng(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Tn5 transposon and its derivatives have been widely used to understand gene functions in Gram-negative bacteria.So far,it has no report that Tn5 is able to transform into Gram-positive bacterium Bacillus pumilus DX01.Here,we generated a Tn5 transposon derivative and successfully transformed it into Bacillus pumilus DX01.1 109 transformants displayed resistance to hygromycin B.To further improve the efficience of Tn5 transformation system in B.pumilus,several electrotransfection parameters including cell inc... |
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| Keywords: | Bacillus pumilus mutation Tn5 transposon electroporation |
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