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体细胞核移植克隆牛的遗传物质鉴定
引用本文:焦飞,颜景斌,李华,黄英,黄淑帧,曾溢滔.体细胞核移植克隆牛的遗传物质鉴定[J].上海交通大学学报(农业科学版),2006,24(3):268-272.
作者姓名:焦飞  颜景斌  李华  黄英  黄淑帧  曾溢滔
作者单位:上海交通大学,上海医学遗传研究所,上海,200040
基金项目:国家高技术研究发展计划(863计划)
摘    要:应用活体取卵(OPU)技术采集遗传背景清晰的卵母细胞作为受体细胞,与不同来源的供体细胞核进行体细胞核移植(SCNT)。通过微卫星DNA及线粒体DNA检测,分别对5头克隆牛核内和核外DNA进行了分析。结果表明克隆牛的微卫星DNA与核供体牛完全一致,而线粒体DNA则全部来源于卵母细胞。

关 键 词:体细胞核移植  活体取卵  微卫星DNA  线粒体DNA
文章编号:1671-9964(2006)03-0268-05
收稿时间:2005-12-12
修稿时间:2005年12月12

Identification of Genetic Materials in Cloned Cattle by Somatic Cell Nuclear Transfer
JIAO Fei,YAN Jing-bin,LI Hu,HUANG Ying,HUANG Shu-zhen,ZENG Yi-tao.Identification of Genetic Materials in Cloned Cattle by Somatic Cell Nuclear Transfer[J].Journal of Shanghai Jiaotong University (Agricultural Science),2006,24(3):268-272.
Authors:JIAO Fei  YAN Jing-bin  LI Hu  HUANG Ying  HUANG Shu-zhen  ZENG Yi-tao
Institution:Shanghai Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040,China
Abstract:The oocytes having specific genetic background recovered by ovum pick up(OPU) technology, were used as recipient cells of somatic cell nuclear transfer(SCNT) to receive donor nuclei from different origins.The nuclear and cytoplasm DNAs of five cloned cattle were detected by microsatellite DNA analysis and mitochondrial DNA analysis,respectively.The results of the present study indicate that the microsatellite DNA of cloned cattle is consistent with the nuclear donor absolutely.However,their mtDNA is originated from corresponding oocytes completely.
Keywords:SCNT  OPU  microsatellite DNA  mtDNA
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