| NAC抑制镉离子诱导的小鼠精子DLD酪氨酸磷酸化作用 |
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| 引用本文： | 文艺,杨强震,李思思,陈俊,刘旭睿,刘桓,李培飞,王立蕊,李新红.NAC抑制镉离子诱导的小鼠精子DLD酪氨酸磷酸化作用[J].上海交通大学学报(农业科学版),2019(2):37-46. |
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| 作者姓名： | 文艺 杨强震 李思思 陈俊 刘旭睿 刘桓 李培飞 王立蕊 李新红 |
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| 作者单位： | 上海交通大学农业与生物学院上海市兽医生物技术重点实验室 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科基字(2014)第2-5号];2017年国家级大学生创新创业训练计划(201710248022);第16期上海交通大学大学生创新实践计划(IPP16123) |
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| 摘 要： | 重金属镉是环境中广泛存在的污染物,对机体生殖系统有很强的毒性作用。镉能诱导精子细胞蛋白二氢硫辛酰胺脱氢酶(DLD)发生酪氨酸磷酸化修饰,但机制尚不清楚。本研究利用蛋白免疫印迹技术探讨镉离子对小鼠生殖系统的毒性作用、镉诱导生殖细胞蛋白发生酪氨酸磷酸化修饰的机理及抗氧化剂N-乙酰-L-半胱氨酸(NAC)对精子的保护作用。结果表明,镉离子能减弱生殖细胞的能量代谢水平,从而抑制睾丸的生长发育并导致产仔数下降;镉离子能特异性诱导精子DLD蛋白发生酪氨酸磷酸化修饰,且与钙离子具有协同作用。此外,NAC可在体外有效抑制DLD蛋白发生酪氨酸磷酸化从而抑制镉的生殖毒性,并恢复精子能量水平和活力参数。本研究从NAC抑制镉离子诱导特异性蛋白磷酸化修饰的角度,揭示NAC保护作用机制,旨在为镉离子生殖毒理学研究及疾病防治提供理论基础。
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| 关 键 词： | 镉 小鼠精子 二氢硫辛酰胺脱氢酶 磷酸化 N-乙酰-L-半胱氨酸 |
| 收稿时间： | 2018/5/16 0:00:00 |
N-acetyl-L-cysteine inhibits tyrosine phosphorylation of dihydrolipoamide dehydrogenase induced by cadmium in mouse spermatozoa |
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| WEN Yi,YANG Qiang-zhen,LI Si-si,CHEN Jun,LIU Xu-rui,LIU Huan,LI Pei-fei,WANG Li-rui and LI Xin-hong.N-acetyl-L-cysteine inhibits tyrosine phosphorylation of dihydrolipoamide dehydrogenase induced by cadmium in mouse spermatozoa[J].Journal of Shanghai Jiaotong University (Agricultural Science),2019(2):37-46. |
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| Authors: | WEN Yi YANG Qiang-zhen LI Si-si CHEN Jun LIU Xu-rui LIU Huan LI Pei-fei WANG Li-rui and LI Xin-hong |
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| Institution: | Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China and Shanghai Key Lab of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | Cadmium (Cd),a widespread pollutant in environment,has toxic effects on organic reproductive system.Cd can induce specific protein (DLD) tyrosine phosphorylation of sperm cells,but its reduction mechanism remains unknown.In our research,western blot was employed to investigate the toxic performances of Cd on reproductive system,the mechanisms of Cd-induced protein tyrosine phosphorylation and the protective effect of antioxidant N-acetyl-L-cysteine (NAC) on sperm.The results showed that Cd inhibited the growth and development of testis and the fertilization ability of sperm cells by decreasing the energy metabolism level of reproductive cells.Cd can specifically induce DLD protein tyrosine phosphorylation and it has synergistic effects with calcium.NAC can effectively inhibit Cd-induced DLD tyrosine phosphorylation and restore the energy metabolism level and motility parameters of sperm to inhibit the reproductive toxicity of Cd in vitro.This research studied the inhibiting effects of NAC on specific protein phosphorylation that was induced by Cd in order to reveal the protective mechanisms of NAC,and provide basic information for reproductive toxicity of Cd and the prevention of Cd-caused diseases. |
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| Keywords: | cadmium mice sperm dihydrolipoamide dehydrogenase (DLD) phosphorylation N-acetyl-L-cysteine (NAC) |
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