| 香水莲花多糖粗提物的除蛋白及抗氧化活性研究 |
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| 引用本文： | 丛恒雪,胡国胜,王文翠,谢先辉,姚雷,卢静.香水莲花多糖粗提物的除蛋白及抗氧化活性研究[J].上海交通大学学报(农业科学版),2018(6):45-49. |
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| 作者姓名： | 丛恒雪 胡国胜 王文翠 谢先辉 姚雷 卢静 |
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| 作者单位： | 上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240 |
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| 基金项目： | 瑞生生物-上海交大芳香植物产业科技示范工程(15H10000536) |
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| 摘 要： | 为了寻找新型天然抗氧化剂,对香水莲花(Nymphaea hybrid)多糖粗提物进行了除蛋白纯化并测定了纯化产物的抗氧化活性。实验结果表明,三氯乙酸法(TCA)的除蛋白效果优于Sevage法和TCA-Sevage法,其蛋白清除率为70.49%,多糖损失率29.24%。TCA法除蛋白后的多糖,对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟自由基都有较强的清除能力,且与浓度呈量效关系,有一定的还原能力,对脂质过氧化抑制效果明显,具有一定的抗氧化活性。
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| 关 键 词： | 香水莲花 多糖 蛋白质 抗氧化活性 |
| 收稿时间： | 2017/9/7 0:00:00 |
Study on protein removal and antioxidant activity of crude polysaccharide from Nymphaea hybrid |
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| CONG Heng-xue,HU Guo-sheng,WANG Wen-cui,XIE Xian-hui,YAO Lei and LU Jing.Study on protein removal and antioxidant activity of crude polysaccharide from Nymphaea hybrid[J].Journal of Shanghai Jiaotong University (Agricultural Science),2018(6):45-49. |
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| Authors: | CONG Heng-xue HU Guo-sheng WANG Wen-cui XIE Xian-hui YAO Lei and LU Jing |
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| Institution: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | The present study compared the deproteinzation methods for crude polysaccharide from Nymphaea hybrid and investigated antioxidant activity of the purified products.The TCA methods gave the best results (with 70.49% protein removal and 29.24% polysaccharide loss) followed by Sevage and Sevage-TCA methods.The product purified by TCA method provided remarkable scavenging effects against DPPH radical and hydroxyl radical(·OH) with a dose dependent manner.Besides,inhibitory effect of the product on lipid peroxidation was observed,showing its antioxidant capacity. |
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| Keywords: | Nymphaea hybrid polysaccharide protein antioxidant activity |
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